Miniaturized bioanalytics to probe the function of membrane proteins
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Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV b ...
Imagine a small integrated biomedical analysis laboratory, connected to your home computer, which would be capable of diagnosing illnesses, a lack of vitamins, or the over-presence of substances from samples of blood, urine or saliva. This hypothetic syste ...
During the last 25 years, display techniques such as phage display have become very powerful tools for protein engineering, especially for the selection of monoclonal antibodies. However, while this method is extremely efficient for affinity-based selectio ...
Recent developments in single-molecule localization techniques using photoactivatable fluorescent proteins have allowed the probing of single-molecule motion in a living cell with high specificity, millisecond time resolution, and nanometer spatial resolut ...
The development of some solid tumors is associated with overexpression of the epidermal growth factor receptor (EGFR) and often correlates with poor prognosis. Near field scanning optical microscopy, a technique with subdiffraction-limited optical resoluti ...
The invention concerns a method for screening compound interaction with transmembrane receptors involved in cellular signal transduction, preferably with G protein coupled receptors, by adding the compound to be analyzed into a medium comprising native inv ...
An emerging concept in cell signaling is the natural role of reactive oxygen species such as hydrogen peroxide (H2O2) as beneficial messengers in redox signaling pathways. The nature of H2O2 signaling is confounded, however, by difficulties in tracking it ...
Metal complexation by dissolved organic matter, as humic acids, is considered to decrease metal bioavailability by lowering the free metal ion concentration. However, dissolved organic matter adsorption on cell surfaces can modify cell membrane properties, ...
The second harmonic generation (SHG) active nanocrystals have been demonstrated as attractive imaging probes in nonlinear microscopy due to their coherent, non-bleaching and non-blinking signals with a broad flexibility in the choice of excitation waveleng ...
Brain-implantable microprobe arrays, 6.5 mm shaft-length, incorporating several recessed Pt microelectrodes (50 mu m x 150 mu m) and an integrated Ag/AgCl reference electrode fabricated by silicon micromachining dry etching techniques (DRIE) are described. ...