Publication
We present a novel method for on-chip competitive dispersion immunoassay (DISIA) which aims at measuring the specificity between fluorescently-labeled antibodies (Ab*) and label-free antigens (Ag). We have fabricated a nanofluidic device that allows fast detection of interaction of antibodies and antigens (< 30s) based on lateral dispersion of fluorescent species in a nanoslit, using small amounts of solution (< 1μl). We measured that doubling the weight of streptavidin protein results in an increase of 30% of the lateral dispersion of fluorescent coupled molecules in the nanoslit, which can be read easily.
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü
Christoph Merten, Jatin Panwar
Julia Schmale, Ivo Fabio Beck, Alireza Moallemi, Margarida Teles Nogueira Rolo