Isolation and in vitro chondrogenic potential of human fetal spine cells
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Stem cell use in bladder tissue engineering is a recently addressed area of investigation that has generated excitement as a novel way to restore and regenerate lost or damaged urinary bladder tissue. The remodelling of smooth muscle plays a significant ro ...
Most tissue engineering approaches include the delivery of reparative cells to a damaged host tissue. Generally these cells are sought to be of a stem cell character since they retain a high potential for proliferation and differentiation into diverse phen ...
Muscle degenerative disorders, such as Duchenne muscular dystrophy, have a profound impact on the quality of life and survival of patients. Transplantation of myoblasts to restore muscle function has been discouraging since these cells die and don't migrat ...
Mesenchymal stem cells (MSC) are a population of multipotent cells that can differentiate into various lineages such as fibroblast, osteoblast, chondrocyte and adipocyte and can be maintained over extended periods in vitro. They are also known to have a su ...
We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue engineering applications. Adult mesenchymal stem cells and adult osteoblasts were included in the study for comparison. Surface markers involved in b ...
In the domain of regenerative medicine research there are many fronts aimed at increasing our understanding of induced pluripotent, embryonic and adult stem cells. Recently a new front has appeared, stem cell niches, the microenvironment that is a crucial ...
Human embryonic stem (hES) cells can self-renew in culture and differentiate into cells of all three germ layers. Identification of regulators of the self-renewal versus differentiation decisions in hES cells will facilitate manipulation of these cells. Se ...
Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity (simple culture conditions), maintenance of differentiated phenotype for primary human cell culture and trans ...
This unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. ...
As primary lymphoid organ, the thymus provides the essential environment for maturation and selection of functional T lymphocytes. Thymic epithelial (TE) cells derive from the endoderm, and more specifically, from the third pharyngeal pouch. The thymic epi ...
