Dimer dissociation of the pore-forming toxin aerolysin precedes receptor binding
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A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent li ...
Sequential stages in the life cycle of the ionotropic 5-HT3 receptor (5-HT3R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intrace ...
Hormone binding nuclear receptors are transcription factors that activate gene transcription by binding to specific DNA sequences in the gene promoter region and then recruiting the necessary coactivator proteins for transcriptional activation. The knowled ...
The ability of organisms, or individual cells, to react to external chemical signals, which are detected and transduced by cell-surface receptors, is crucial for their survival. These receptors are the targets of the majority of clinically used medicines. ...
Fluorescence Correlation Spectroscopy (FCS) is a suitable technique to investigate biomol. interactions. The advantage of FCS lies in its wide dynamic range in the time and concn. domain, its sensitivity, and thus in the small amt. of material needed. The ...
The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...
We have expressed the 5-HT3 receptor with two transient expression sytems: the Semliki Forest virus and the HEK293EBNA. Both systems allow the rapid expression of functional receptor protein at very high level: up to 107 receptors per cell. Large scale pro ...
A theoretical model describing the attachment and cytoskeletal coupling of microspheres to the dorsal surface of motile cells was developed. Integral membrane receptors beneath a ligand-coated microsphere are allowed to be either free, attached to the micr ...
A fluorescently labeled ligand for the 5-HT3 serotonin receptor was synthesized and its sub-nanomolar affinity for the purified, detergent solubilized receptor was measured. The change in the ligand's fluorescence upon receptor binding was used to directly ...
G protein-coupled receptors (GPCRs) constitute an abundant family of membrane receptors of high pharmacol. interest. Cell-based assays are the predominant means of assessing GPCR activation, but are limited by their inherent complexity. Functional mol. ass ...