Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresis
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The Off-Gel™ technology is an isoelectric focusing (IEF) fractionation method which enables the purification of one or several proteins in a solution placed on an immobilised pH gradient (IPG) gel. The present thesis is devoted to its characterisation and ...
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Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...
Membrane-associated proteins of Mycobacterium tuberculosis offer a challenge, as well as an opportunity, in the quest for better therapeutic and prophylactic interventions against tuberculosis. The authors have previously reported that extraction with the ...
