Coupling of organotypic brain slice cultures to silicon-based arrays of electrodes
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Understanding how the information is coded in large neuronal networks is one of the major challenges for neuroscience. A possible approach to investigate the information processing capabilities of the neuronal ensembles is given by the use of dissociated n ...
Synaptic contacts formed by the axon of a neuron on its own dendrites are known as autapses. Autaptic contacts occur frequently in cultured neurons and have been considered to be aberrant structures. We examined the regular occurrence, dendritic distributi ...
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In this study we examined the passive biocompatibility of a three-dimensional microelectrode array (MEA), designed to be coupled to organotypic brain slice cultures for multisite recording of electrophysiological signals. Hippocampal (and corticostriatal) ...
Several multi-electrode array devices integrating planar metal electrodes were designed in the past 30 years for extracellular stimulation and recording from cultured neuronal cells and organotypic brain slices. However, these devices are not well suited f ...
Abstract—Mapping neuronal networks activity of in-vitro cell cultures with microelectrode array (MEA) based systems, providing a spatial resolution down to cellular/sub-cellular levels and an adequate temporal resolution for resolving action potential prop ...
A large number of studies on neuronal physiology and plasticity have provided a detailed picture of the molecular machinery underlying the modulation of neuronal activity. On the contrary, the mechanisms controlling properties of complex neuronal networks ...
Technological innovations in the fields of biomedical optics and electronics have lead to an extremely high level of miniaturization. These steps opened important perspectives to interface optoelectronic instruments with cellular systems. In this frame, a ...
The cellular mechanism by which acetylcholine (ACh) potentiates neuronal responses to N-methyl-D-aspartate (NMDA) was investigated in CA1 neurones of hippocampal slices using current- and voltage-clamp techniques. 2. Loading cells with 5'-guanylylimidod ...
Since their introduction, Micro-Electrode Arrays (MEAs) have been exploited as devices providing distributed information about learning, memory and information processing in a cultured neuronal network, thus changing the field of view from single cell leve ...