Publication

Super-resolved position and orientation of fluorescent dipoles

Publications associées (32)

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Several micron-range measurements with sub-nanometric resolution by the use of dual-wavelength digital holography and vertical scanning

Christian Depeursinge, Tristan Colomb, Jonas Kühn

Reflection digital holographic microscopy (DHM) is a very powerful technique allowing measuring topography with a sub-nanometer axial resolution from a single hologram acquisition. But as most of interferometer methods, the vertical range is limited to hal ...

SPIE2009

Super-resolved position and orientation from defocused images of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore’s underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolu ...

2009

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

OSA2009

Steerable filters for orientation estimation and localization of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

Steerable Filters for Orientation Estimation and Localization of Fluorescent Dipoles

Michaël Unser, Theo Lasser, François Aguet

IEEE2009

Bow-tie optical antenna probes for single-emitter scanning near-field optical microscopy

Philippe Flückiger

A method for the fabrication of bow-tie optical antennas at the apex of pyramidal Si3N4 atomic force microscopy tips is described. We demonstrate that these novel optical probes are capable of sub-wavelength imaging of single quantum dots at room temperatu ...

2007

Tunable nanowire nonlinear optical probe

Aleksandra Radenovic

One crucial challenge for subwavelength optics has been the development of a tunable source of coherent laser radiation for use in the physical, information and biological sciences that is stable at room temperature and physiological conditions. Current ad ...

2007

Theory of resonantly enhanced near-field imaging

Demetri Psaltis

We investigate the fundamental issues of power transfer and far-field retrieval of subwavelength information in resonantly enhanced near-field imaging systems. It is found that high-quality resonance of the imaging system, such as that provided by dielectr ...

2007

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