Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.
Stéphanie Lacour, Outman Akouissi, Emilio Fernández Lavado, Massimo Mariello, Kutay Sagdic
Lijing Xin, Cristina Ramona Cudalbu, Mario Gaetano Lepore, Marta Lai
Martin Ehrbar, Queralt Vallmajó Martín