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Optical tomography with digital holographic microscopy

Publications associées (33)

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Digital Phase Reconstruction via Iterative Solutions of Transport-of-Intensity Equation

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We develop a variational algorithm for reconstructing phase objects from a series of bright field micrographs. Our mathematical model is based on the transport-of-intensity equation (TIE), which links the phase of a complex field to the axial derivative of ...
Ieee2014

Digital Phase Reconstruction via Iterative Solutions of Transport-of-Intensity Equation

Michaël Unser, Emrah Bostan, Stamatios Lefkimmiatis, Emmanuel Augustin Julien Froustey

We develop a variational algorithm for reconstructing phase objects from a series of bright field micrographs. Our mathematical model is based on the transport-of-intensity equation (TIE), which links the phase of a complex field to the axial derivative of ...
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Marker-free phase nanoscopy

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We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...
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Resolution and Complex Deconvolution in Holographic Microscopy

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Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...
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Digital holographic microscopy for the cytomorphological imaging of cells under zero gravity

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Digital holographic microscopy (DHM) has been gaining interest from cell biology community because of its label free nature and quantitative phase signal output. Besides, fast shutter time, image reconstruction by numerical propagation of the wave fields, ...
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2012

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

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We present in this PhD thesis work various applications of digital holographic microscopy (DHM), an imaging technique based on coherent illumination which enables the recovery of the full complex wavefront, i.e. the amplitude and phase of a wave field whic ...
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Complex index refraction tomography with sub ?/6-resolution

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The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...
2011

Realistic 3D coherent transfer function inverse filtering of complex fields

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We present a novel technique for three-dimensional (3D) image processing of complex fields. It consists in inverting the coherent image formation by filtering the complex spectrum with a realistic 3D coherent transfer function (CTF) of a high-NA digital ho ...
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