Digital holographic microscopy for the cytomorphological imaging of cells under zero gravity

Digital holographic microscopy (DHM) has been gaining interest from cell biology community because of its label free nature and quantitative phase signal output. Besides, fast shutter time, image reconstruction by numerical propagation of the wave fields, and numerical compensation of the aberrations are other intrinsic advantages of this technique that can be explored for harsh imaging conditions. In the frame of this work, a transmission type DHM is developed with a decoupled epifluorescence microscopy mode for cytomorphological monitoring under zero gravity and hyper gravity. With the implemented automatic post processing routines, real time observation of the cell morphology is proven to be feasible under the influence of mechanical disturbances of zero gravity platforms. Post processing of holograms is composed from dynamic numerical compensation of holograms, robust autofocusing and phase image registration. Experiments on live myoblast cells are carried out on two different platforms; random positioning machine (RPM), a ground base microgravity simulation platform, and parabolic flight campaign (PFC), a fixed wing plane flight providing short durations of alternating gravity conditions. Results show clear perinuclear phase increase. During seconds scale microgravity exposure, measurable scale morphological modifications are observed with the accumulated effect of repetitive exposures and short breaks.

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Concepts associés (2)

Digital holographic microscopy

Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The image forming lens in traditional microscopy is thus replaced by a computer algorithm.

Gravitation

La gravitation, l'une des quatre interactions fondamentales qui régissent l'Univers, est l' physique responsable de l'attraction des corps massifs. Elle se manifeste notamment par l'attraction terrestre qui nous retient au sol, la gravité, qui est responsable de plusieurs manifestations naturelles; les marées, l'orbite des planètes autour du Soleil, la sphéricité de la plupart des corps célestes en sont quelques exemples. D'une manière plus générale, la structure à grande échelle de l'Univers est déterminée par la gravitation.