Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics
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Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. ...
Recombinant proteins are gaining in importance for therapeutic applications. The proteins are expressed in stable cell lines, within which recombinant DNA has incorporated into the host cells genome. Identification and isolation of extremely high producers ...
With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...
Fluorescence resonance energy transfer (FRET) allows the user to investigate interactions between fluorescent partners. One crucial issue when calculating sensitized emission FRET is the correction for spectral bleed-throughs (SBTs), which requires to calc ...
Using the combinatorial material synthesis approach, materials libraries can be produced in one experiment that contain up to several thousand samples on a single substrate. In order to identify optimized materials in an efficient way using screening metho ...
Cyclosporins are a family of hydrophobic cyclic undecapeptides produced by the fungus Tolypocladium niveum. The main metabolite cyclosporin A (CsA, see Scheme, R = R' = H) is the first line drug currently used to prevent rejection of organ transplants. Its ...
A review of recent developments in the labeling of fusion proteins with synthetic mols. in the living cell or in complex mixts. The most commonly used tags are presented and then focuses on a new class of tags that are not limited to their genetically enco ...
Methods for functionalising polymers for tissue engineering are disclosed in which a polymer matrix is activated to bind to biological materials such as cells by covalently bonding a hydrophilic polymer to carboxy groups of a polymer matrix. The hydrophili ...
We have measured fluorescence resonance energy transfer (FRET) between a fluorescent antagonist, bound to the purified detergent-solubilized serotonin type 3 receptor, and a lipophilic acceptor probe partitioned into the micelle surrounding the detergent-s ...
Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fl ...