Fura-2FF-based calcium indicator for protein labeling
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A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...
Micro-optical systems based on refractive microlenses are investigated. These systems are integrated on a chemical chip. They focus an excitation beam into the detection volume (microliter or even submicroliter scale) and collect the emitted light from flu ...
Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
Fluorescent dye tracers are used to show concentration maps of tracer fronts, plumes and pathways on soil profiles and in monolith cross sections. The physical process of fluorescence from the exposed soil surfaces must be understood in order to retrieve a ...
A general approach for the sequential labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifi ...
The past few years we have witnessed a tremendous surge of interest in so-called array-based miniaturised analytical systems due to their value as extremely powerful tools for high-throughput sequence analysis, drug discovery and development, and diagnosti ...
Steady-state and picosecond (ps) fluorescence studies of wild-type bacteriorhodopsin (wt-bR) and of a nonisomerizing analog locked in the all-trans configuration have been performed. Extending earlier work done by femtosecond absorption spectroscopy, we ob ...
Micro-optical systems based on refractive microlenses are investigated. These systems are integrated on a chemical chip. They focus an excitation beam into the detection volume (microliter or even submicroliter scale) and collect the emitted light from flu ...
Highly structured fluorescence and fluorescence excitation spectra of C70 in Ne matrixes are reported. Fluorescence from both S1 and S2 states is obsd., and these are identified as being of A2' and E1' and have energies of 15,524 cm-1 and 15,691 cm-1, resp ...
A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled recept ...
