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Rapid purification of active gamma-secretase, an intramembrane protease implicated in Alzheimer's disease

Publications associées (46)

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Phage Pierces the Host Cell Membrane with the Iron-Loaded Spike

Petr Leiman, Christopher Browning

Bacteriophages with contractile tails and the bacterial type VI secretion system have been proposed to use a special protein to create an opening in the host cell membrane during infection. These proteins have a modular architecture but invariably contain ...

2012

Automation of image processing in electron crystallography

Henning Paul-Julius Stahlberg

Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D c ...

Humana Press2012

Merging of image data in electron crystallography

Henning Paul-Julius Stahlberg

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the re ...

Humana Press2012

CitS from Klebsiella pneumoniae acts as a secondary symporter of citrate and sodium ions across the inner membrane of the host. The protein is the best characterized member of the 2-hydroxycarboxylate transporter family, while no experimental structural in ...

Elsevier BV2012

Generation of monoclonal antibody fragments binding the native γ-secretase complex for use in structural studies

Lorène Aeschbach, Patrick Fraering, Mitko Dimitrov, Claude André Schweizer

A detailed understanding of γ-secretase structure is crucially needed to elucidate its unique properties of intramembrane protein cleavage and to design therapeutic compounds for the safe treatment of Alzheimer's disease. γ-Secretase is an enzyme complex c ...

Amer Chemical Soc2012

Alzheimer's Disease-Linked Mutations in Presenilin-1 Result in a Drastic Loss of Activity in Purified gamma-Secretase Complexes

Lorène Aeschbach, Patrick Fraering, Jemila Houacine

Background: Mutations linked to early onset, familial forms of Alzheimer's disease (FAD) are found most frequently in PSEN1, the gene encoding presenilin-1 (PS1). Together with nicastrin (NCT), anterior pharynx-defective protein 1 (APH1), and presenilin en ...

Public Library of Science2012

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Ruthenium complexes with bridging dicarboxylate ligands were combined with 1,2-di-4-pyridylethylene (dpe), 2,4,6-tri-4-pyridyltriazine (4-tpt), or 2,4,6-tri-3-pyridyltriazine (3-tpt) to give a tetranuclear rectangle or hexanuclear coordination cages. The c ...

2012

Alzheimer's disease (AD) is a devastating neurodegenerative disorder which severely impairs cognitive functions by triggering neuronal cell death and synaptic loss, and finally leads the patients to death. Two main histopathological hallmarks can be found ...

EPFL2011

Automatic recovery of missing amplitudes and phases in tilt-limited electron crystallography of two-dimensional crystals

Henning Paul-Julius Stahlberg

Electron crystallography of 2D protein crystals provides a powerful tool for the determination of membrane protein structure. In this method, data is acquired in the Fourier domain as randomly sampled, uncoupled, amplitudes and phases. Due to physical cons ...

American Physical Society (APS)2011

Preparation of 2D Crystals of Membrane Proteins for High-Resolution Electron Crystallography Data Collection

Henning Paul-Julius Stahlberg, Radosav Svetozara Pantelic

Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscop ...

Elsevier2010