Publication

Quantitative phase restoration by direct inversion using the optical transfer function

Publications associées (32)

Cellular dynamics explored with digital holographic microscopy

Obtaining high resolution 3D quantitative images is critical for elucidating the relationship between cell dynamics and physiological or pathological processes. However the feasibility of such cellular dynamic studies is limited by the resolution of the 3D ...

EPFL2008

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Florian Charrière

The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...

EPFL2007

Automatic procedure for aberrations compensation in digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Nicolas Aspert, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

Digital Holographic Microscopy (DHM) is a powerful imaging technique allowing, from a single amplitude image (amplitude and phase), reflected acquisition (hologram), the reconstruction of the entire complex wave front by or transmitted through an object. B ...

SPIE2006

Biological cell (pollen grain) refractive index tomography with digital holographic microscopy

Christian Depeursinge, Etienne Cuche, Pierre Marquet, Florian Charrière

This paper present a novel approach to perform the tomography of biological specimen based on Digital Holographic Microscopy (DHM). A hologram results from the interference between a reference wave and an object wave reflected from or transmitted through a ...

SPIE2006

Complex unltrahigh resolution Fourier domain optical coherence tomography

Theo Lasser, Rainer Leitgeb, Adrian Bachmann

Fourier domain optical coherence tomography (FDOCT) is a high speed imaging technique with high axial resolution in the micro-meter-scale range combined with a high sensitivity allowing to probe 3D volumes of weakly back- scattering biological tissues in-v ...

SPIE2006

Phase estimation methods and their application to holographic interferometry

Rajesh Langoju

Phase of the interference fringe pattern is known to convey important information in optical metrology. Typically, phase measurement using temporal techniques involves incorporating a piezoelectric device (PZT) in one arm of the interferometer for shifting ...

EPFL2006

Measurement and interpretation of the 3D amplitude point spread function of lenses and microscope objectives

The intensity point spread function (IPSF) is most often used to characterize optical imaging systems. For some imaging applications such as digital holographic microscopy the 3D amplitude point spread function (APSF) more completely describes an optical s ...

EPFL2005

Real-time radiology in the microscale

Giorgio Margaritondo

We present several examples of the applications of a novel microradiology approach based on unmonochromatized synchrotron x-rays. The approach - mostly implemented on the ICPCIR (International Consortium on Phase Contrast Imaging and Radiology) Beamline at ...

2005

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Magistretti, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Benjamin Rappaz, Pierre Marquet

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsew ...

2005

3D imaging of surfaces and cells by numerical reconstruction of wavefronts in Digital Holography applied to transmission and reflection microscopy

Pierre Magistretti, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Pierre Marquet, Mihaela Marian

We report on a numerical method for the reconstruction of wavefronts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the pr ...

IEEE2002

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