Publication
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of beta 2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
Maartje Martina Cornelia Bastings, Diana Kornelia Morzy
Giovanni D'Angelo, Laura Capolupo, Anthony Vocat, Riccardo Rizzo
Philippe Renaud, Horst Vogel, Thamani Dahoun, Margaux Catherine Marie Duchamp