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Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do ...
Bloch surface waves (BSWs) are surface electromagnetic waves excited at the interface between a truncated periodic dielectric multilayer and a surrounding medium. This particular solution to the Maxwell equations in stratified media has been known since th ...
Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...
A technique that allows direct optical imaging of nanostructures and determines quantitatively geometric nanofeatures beyond the classical diffraction limit by using high-refractive index glass microspheres is introduced. The glass microsphere is put on a ...
We propose the use of a high-refractive index glass microsphere in combination with a conventional fluorescence microscope for imaging sub-cellular organelles and biomolecules. The microsphere is placed on a sample that is immersed in water, collects the n ...
We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...
Optical microscopy has become a major non- or minimally invasive tool for investigating living organisms such as cells, tissues or small animals. Being such an essential tool for research and because lacking knowledge and insight in the working principles ...
We present the fabrication and optical characterization of nano-scale solid immersion lenses (nano-SILs) with sizes down to a subwavelength range. Submicron-scale cylinders fabricated by electron-beam lithography (EBL) are thermally reflowed to form a sphe ...
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2012
We report on the advanced optical characterizations of microfabricated solid immersion lenses with 2-mu m diameter, operating at lambda = 642 nm. The main feature, the spot size reduction, has been investigated by applying a focused Gaussian beam of NA = 0 ...
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2012
A technique that allows direct optical imaging of nanostructures and determines quantitatively geometric nanofeatures beyond the classical diffraction limit by using high-refractive index glass microspheres is introduced. The glass microsphere is put on th ...
