Production of a thermostable fumarase in Saccharomyces cerevisiae for the bioconversion of fumarate to L-malate
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The bioconversion of L-phenylalanine (L-Phe) to 2-phenylethanol (PEA) by the yeast Saccharomyces cerevisiae is limited by the toxicity of the product. PEA extn. by a sep. org. phase in the fermenter is the ideal in situ product recovery (ISPR) technique to ...
The dynamic behavior of a continuous culture of Saccharomyces cerevisiae subjected to a sudden increase in the diln. rate has been successfully modelled for anaerobic growth on glucose, and for aerobic growth on acetate, on ethanol, and on glucose. The cat ...
Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored proteins involves the action of a GPI trans-amidase, which replaces the C-terminal GPI signal sequence (GPI-SS) of the primary translation product with a preformed GPI lipid. The transamidation de ...
The authors present here a novel approach to induce protein dimerization in living cells through covalent labeling of fusion proteins with ligands which are interacting with other proteins. The covalent labeling is based on the reaction of the DNA repair p ...
This paper describes an improvement of a method for continuous calibration of the global heat transfer coeff., recently described in literature [A. Tietze, Moglichkeiten und Grenzen der Temperaturschwingungskalorimetrie, Doctoral Thesis, Technishe Universi ...
A process is provided for the in-situ extn. of a fermn. product esp. for products which result from the transformation of an amino acid and which are sol. in the fermn. broth. The sol. product is then removed from aq. phase by extn. into an org. phase wher ...
In Saccharomyces cerevisiae, the expression of invertase, which is the hydrolyzing enzyme of sucrose, is controlled by the presence of monosaccharides, such as glucose and fructose, and referred to as carbon catabolite repression. To date, efforts have bee ...
The invention provides methods and materials related to the prodn. of org. products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and metho ...
A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. ...
The inhibitory impacts of the bioconversion of L-phenylalanine (L-Phe) to 2-phenylethanol (PEA), a very important natural aroma compd., on the metab. of Saccharomyces cerevisiae Giv 2009 were investigated in batch and chemostat cultures. The bioconversion ...