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High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Publications associées (81)

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3D printed large amplitude torsional microactuators powered by ultrasound

Mahmut Selman Sakar, Mehdi Ali Gadiri, Junsun Hwang, Amit Yedidia Dolev

Here, we introduce a design, fabrication, and control methodology for large amplitude torsional microactuators powered by ultrasound. The microactuators are 3D printed from two polymers with drastically different elastic moduli as a monolithic compliant me ...

2024

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. Quantitative ph ...

NATURE PORTFOLIO2022

Phase Reconstruction of Low-Energy Electron Holograms of Individual Proteins

Klaus Kern, Stephan Rauschenbach, Sven Alexander Szilagyi, Hannah Julia Ochner

Low-energy electron holography (LEEH) is one of the few techniques capable of imaging large and complex three-dimensional molecules, such as proteins, on the single molecule level at subnanometer resolution. During the imaging process, the structural infor ...

AMER CHEMICAL SOC2022

Lock-in amplified differential phase contrast

Christophe Moser, Timothé Laforest, Damien Claude-Marie Loterie, Chiara Bonati

We introduce a lock-in method to increase the phase contrast in incoherent Differential Phase Contrast (DPC) imaging. The use of a smart pixel detector with in-pixel signal demodulation, paired with synchronized illumination, provides the basis of a bit-ef ...

SPIE-INT SOC OPTICAL ENGINEERING2022

Lock-in incoherent differential phase contrast imaging

Christophe Moser, Timothé Laforest, Damien Claude-Marie Loterie, Chiara Bonati

We introduce a lock-in method to increase the phase contrast in incoherent differential phase contrast (DPC) imaging. This method improves the phase sensitivity by the analog removal of the background. The use of a smart pixel detector with in-pixel signal ...

CHINESE LASER PRESS2022

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Direct visualization is often sought to elucidate flow patterns and validate models to predict the filling kinetics during processes whereby a liquid resin infiltrates a textile porous preform. Here, X-ray phase contrast interferometry is evaluated to imag ...

2021

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Image relighting is attracting increasing interest due to its various applications. From a research perspective, image relighting can be exploited to conduct both image normalization for domain adaptation, and also for data augmentation. It also has multip ...

2021

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A new approach to optical diffraction tomography (ODT) based on intensity measurements is presented. By applying the Wolf transform directly to intensity measurements, we observed unexpected behavior in the 3D reconstruction of the sample. Such a reconstru ...

OPTICAL SOC AMER2021

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

High-resolution interference microscopy of binary phase diffractive optical elements

Toralf Scharf, Dong Cheon Kim, Michail Symeonidis

In this work, we apply the high-resolution interference microscopy technique to investigate the intensity and phase of light transmitted through a binary phase element. Using a combination of simulation and experimental results, we identify specific featur ...

OPTICAL SOC AMER2019