An All-Digital, Time-gated 128x128 SPAD Array for On-chip, Filter-less Fluorescence Detection
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We present a CMOS image sensor with deep-subwavelength, digital 1-T pixels designed for use in a digital film concept known as Gigavision (GV) camera. In GV, the levels of gray in the picture are derived from the concentration of activated pixels. Activate ...
Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl2(en)], [{RhCl2Cp*}2], and [{RuCl2(p-cymene)} 2] were combined with six different fluorescent dyes to build a cross-reactiv ...
Elucidation of in vivo cholesterol transport and its aberrations in cardiovascular diseases requires suitable model organisms and the development of appropriate monitoring technology. We recently presented a new approach to visualize transport of the intri ...
Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...
We propose a new image device called gigavision camera. The main differences between a conventional and a gigavision camera are that the pixels of the gigavision camera are binary and orders of magnitude smaller. A gigavision camera can be built using stan ...
Silicon is an excellent detector material for electromagnetic radiation in the wavelength range of 0.1 to 1000 nm. In the visible spectral range (400-700 nm), external quantum efficiencies approaching 100% are obtained. When combined with the amazing minia ...
A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...
Time-correlated single photon counting techniques have recently established themselves in numerous fields of research. In particular, for fluorescence lifetime imaging, time-correlated single photon counting is often considered the absolute reference in te ...
We present a rapid and flexible framework for the accurate calculation of the detection efficiency of fluorescence emission in isotropic media as well as in the vicinity of dielectric or metallic interfaces. The framework accounts for the dipole characteri ...