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Visualizing Biochemical Activities in Living Cells through Chemistry

Publications associées (56)

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Development of Prototype UrbanSim Models

Michel Bierlaire

UrbanSim is an integrated transportation land-use model that has been under development since the late 1990s. It has received a significant amount of attention in the integrated modeling community. It is well known for its disaggregated approach. A number ...

2010

How to obtain labeled proteins and what to do with them

Kai Johnsson, Marlon Hinner

We review new and established methods for the chemical modification of proteins in living cells and highlight recent applications. The review focuses on tag-mediated protein labeling methods, such as the tetracysteine tag and SNAP-tag, and new developments ...

Elsevier2010

Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore

Kai Johnsson, Mako Kamiya

We introduce here a new class of BODIPY-based Ca2+ indicators which can be derivatized with biological ligands that permit the localization of the indicators in living cells. The underivatized BODIPY-based Ca2+ indicator (BOCA-1) shows a 250-fold increase ...

American Chemical Society2010

Quantitative microstructural characterisation of cement paste submitted to different moderate temperature and loading conditions

Karen Scrivener, Emmanuel Gallucci

The temperature is known to influence the strength and durability of concrete. Temperature has also a great influence on the creep of cementitious materials. Previous investigations have not provided a complete understanding of these effects; in particular ...

2009

Visualizing biochemical activities in living cells

Kai Johnsson

Autofluorescent proteins have become indispensable in our quest to visualize molecular events in living cells. Further progress in the visualization and quantification of all biochemical activities of the cell will require the introduction of additional an ...

2009

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

Kai Johnsson, Marlon Hinner, Matthias Albert Brun, Kui Thong Tan

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate Such fluorescent sensor proteins lack. generality, as they require a protein that ...

2009

Development of prototype UrbanSim models

Michel Bierlaire, Ricardo Daniel Hurtubia González

2008

Development of prototype UrbanSim models

Michel Bierlaire, Ricardo Daniel Hurtubia González

2008

Development of Prototype UrbanSim Models

Michel Bierlaire

UrbanSim is an integrated transportation land-use model that has been under development since the late 1990s. It has received a fair bit of attention in the integrated modeling community. Its notoriety comes primarily from its disaggregated approach. A num ...

2008

Proteins in Mesoporous Silicates

Liang Qiao, Yun Liu

Mesoporous silicates (MPS) provide a means of immobilizing proteins and enzymes in a stable environment while retaining physiological function. A systematic methodology of assessing the potential of immobilising a given protein on to MPS has been developed ...

American Chemical Society2008