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Second harmonic phase microscopy of collagen fibers

Publications associées (45)

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BigNeuron: Large-Scale 3D Neuron Reconstruction from Optical Microscopy Images

Sean Lewis Hill

Understanding the structure of single neurons is critical for understanding how they function within neural circuits. BigNeuron is a new community effort that combines modern bioimaging informatics, recent leaps in labeling and microscopy, and the widely r ...

Elsevier2015

Optical microscopy for biology and life sciences

Theo Lasser, Marcel Leutenegger

Optical microscopy has become a major non- or minimally invasive tool for investigating living organisms such as cells, tissues or small animals. Being such an essential tool for research and because lacking knowledge and insight in the working principles ...

Laboratoire d'Optique Biomédicale, EPFL2014

DEA-based deformable cell culture system

Herbert Shea, Cristina Martin Olmos, Samuel Rosset, Alexandre Poulin, Martha Liley

We present a deformable cell culture system based on dielectric elastomer actuator (DEA). Understanding how the mechanical environment can affect cells functions could lead to significant advances in diseases diagnosis and drug development. Most available ...

2014

High throughput second harmonic imaging for label-free biological applications

Pierre Magistretti, Sylvie Roke, Aleksandra Radenovic, Carlos Alberto Macias Romero, Orly Bagunu Tarun, Pierre Marquet, Pascal Jourdain, Vitalijs Zubkovs, Marie Eve Pascale Didier

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

Sum frequency and second harmonic generation from the surface of a liquid microjet

Sylvie Roke, Nikolay Smolentsev, Yixing Chen, Kailash Chandra Jena

The use of a liquid microjet as a possible source of interest for Second Harmonic Generation (SHG) and Sum Frequency Generation (SFG) spectroscopy is examined. We measured non-resonant SHG scattering patterns from the air/water interface of a microjet of p ...

Amer Inst Physics2014

Developing luminescent Brownian probes for near-field investigations of the intracellular environment

Flavio Maurizio Mor

Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...

EPFL2013

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Optical microscopy and transmission electron microscopy have been used to investigate the morphology of polylactide (PLA)/microfibrillated cellulose (MFC) composites prepared by: compression molding of wet-comingled MFC and PLA latex or powder, twin-screw ...

Springer Berlin Heidelberg2013

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We demonstrate morphology-dependent second-harmonic generation (SHG) from InAs V-shaped nanomembranes. We show SHG correlation with the nano-wing shape and size, experimentally quantify the SHG efficiency, and demonstrate a maximum SHG enhancement of about ...

Royal Society of Chemistry2013

Resolution and Complex Deconvolution in Holographic Microscopy

Yann Jérôme Michel Pascal Cotte

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

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In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

SPIE2011