Publication
Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Publications associées (79)
Molecular mechanism of plasmid elimination by the DdmDE defense system
Melanie Blokesch, Sandrine Stutzmann, David William Adams, Laurie Righi
Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...
2024Sequence-dependent structural properties of B-DNA: what have we learned in 40 years?
John Maddocks, Daiva Petkeviciute, Rahul Sharma, Marco Pasi, Paolo Carloni
The structure of B-DNA, the physiological form of the DNA molecule, has been a central topic in biology, chemistry and physics. Far from uniform and rigid, the double helix was revealed as a flexible and structurally polymorphic molecule. Conformational ch ...
London2021Structural and DNA binding properties of mycobacterial integration host factor mIHF
Stewart Cole, David Lyndon Emsley, Giovanni Dietler, Luciano Andres Abriata, Nina Theres Odermatt, Moreno Lelli, Jérémie Piton, Aleksandre Japaridze, Rajkumar Singh
In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted ...
2020MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering
Christian Iseli, Nicolas Mermod, Thomas Junier, Samuel Neuenschwander, Ioannis Xenarios
Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous r ...
2017Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Florian Maria Wurm, David Hacker, Sowmya Balasubramanian
Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...
American Chemical Society2016Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak
Transient gene expression (TGE) in human embryonic kidney (HEK-293) and Chinese hamster ovary (CHO) cells is a well-established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L or more, the amo ...
American Chemical Society2015Study of transposon-mediated cell pool and cell line generation in CHO cells
The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...
EPFL2015Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova, Sowmya Balasubramanian
Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...
Elsevier2015Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling
Giovanni Dietler, Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak, Aleksandre Japaridze, Robert Sebastian Kwapich
DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated from ...
Oxford University Press2015Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sowmya Balasubramanian
Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amou ...
American Chemical Society2015