Publication

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells

Publications associées (47)

The tracing of neuronal cell lineages is critical to our understanding of cellular diversity in the CNS. This protocol describes a fluorescence birth-dating technique to label, track and isolate isochronic cohorts of newborn cells in the CNS in vivo in mou ...

NATURE PUBLISHING GROUP2018

Thioredoxins (Trxs) are antioxidant proteins that are conserved among all species. These proteins have been extensively studied and perform reducing reactions on a broad range of substrates. Here, we identified Caulobacter crescentus Trx1 (CCNA_03653; CcTr ...

2018

Quantitative analysis of SOX2 and OCT4 expression in pluripotency and differentiation

Daniel Strebinger

Embryonic stem (ES) cells have the capacity to give rise to all cell types of the adult organism and can be used as a model to study early cell fate decisions as they closely recapitulate in vivo events. The M and G1 phases have been suggested to be the ke ...

EPFL2018

Mechanical and Functional Study of Cell Physiology at the Nanoscale

Pascal Damian Odermatt

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

How cells grow and divide is a question that was raised more than 100 years ago. Despite years of research and in-depth knowledge of several molecular mechanisms, the complete system that regulates and coordinates both of those activities within Schizosacc ...

EPFL2014

The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production

Dominique Terry Monteil Jr

The aim of the pharmaceutical industry is to manufacture complex therapeutic proteins or biologics to treat human diseases. The manufacture of biologics requires a significant amount of financial and infrastructure resources. For this reason, efforts are b ...

EPFL2014

Equilibrium self-assembly relies on the relaxation of disordered mixtures of building blocks towards an ordered ground state. The main drawback of this traditional approach lies in the kinetic traps that often interrupt the progression of the system toward ...

Nature Publishing Group2013

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

Microtechnologies to Map the Fate of Single Stem Cells and their Progeny

Stefan Kobel

Since stem cells have the unique ability to produce more of themselves (i.e. to "self-renew") and to generate specialized tissue cells, they are an ideal source of cells for regenerative medicine and in vitro tissue models. In order to fully exploit this p ...

EPFL2012

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