A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells
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Most tissue engineering approaches include the delivery of reparative cells to a damaged host tissue. Generally these cells are sought to be of a stem cell character since they retain a high potential for proliferation and differentiation into diverse phen ...
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BACKGROUND: Acquisition of lineage-specific cell cycle duration is a central feature of metazoan development. The mechanisms by which this is achieved during early embryogenesis are poorly understood. In the nematode Caenorhabditis elegans, differential ce ...
Mitotic exit integrates the reversal of the phosphorylation events initiated by mitotic kinases with a controlled cytokinesis event that cleaves the cell in two. The mitotic exit network (MEN) of budding yeast regulates both processes, whereas the fission ...
The nutritional value of diatoms for grazers has been frequently addressed in experiments. However, little is known about the effects of culture conditions on the quality of algae as food for higher trophic levels. We examined the fatty acid composition of ...
Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used ato ...
Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...
Today, more than 30 products using a manufacturing process based on mammalian cell culture have been approved for human therapy. Most of these products are currently supplied with stirred tank bioreactors operated in batch or fed-batch mode. However, the b ...
