Publication

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

Publications associées (35)

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We present a physically-based computational model of the light sheet fluorescence microscope (LSFM). Based on Monte Carlo ray tracing and geometric optics, our method simulates the operational aspects and image formation process of the LSFM. This simulated ...

BioMed Central2015

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Joan Teixidor

A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

A micro particle shadow velocimetry (mu PSV) technique to measure flows in microchannels

John Richard Thome, Giulia Tagliabue, Navid Borhani, Sepideh Khodaparast

A micro particle shadow velocimetry (mu PSV) system based on back-lit illumination and forward scatter observation of light from non-fluorescent particles has been developed. Relatively high luminous efficiencies and particle image contrasts were achieved ...

Springer-Verlag2013

Programmable parylene-C bonding layer fluorescence for storing information on microfluidic chips

Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik

We demonstrate data storage on glass/silicon microfluidic devices fabricated using parylene-C as a bonding layer. In particular, we report intermediate parylene-C bonding layer fluorescence (iPBLF) and its use as an on-chip medium for data storage by dynam ...

Royal Society of Chemistry2013

Functional and Structural Imaging with Optical Coherence Microscopy

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Simultaneous measurement of liquid absorbance and refractive index using a compact optofluidic probe

Maurine Malak Karam Awadallah

We present a novel optical technique for simultaneously measuring the absorbance and the refractive index of a thin film using an infrared optofluidic probe. Experiments were carried on two different liquids and the results agree with the bibliographical d ...

Royal Society of Chemistry2013

Self-assembled melamine microlens arrays for immunofluorescence enhancement

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Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...

2013

Dynamically programmable parylene-C bonding layer fluorescence for re-writable data storage on a microfluidic chip

Martinus Gijs, Ata Tuna Ciftlik

Wafer bonding using parylene-C to Si, SiO2 and SiN surfaces has recently been shown to enable high pressure-resistant microfluidic devices with metallization and CMOS-integration capability. Here, we report characterization of intermediate parylene-C bondi ...

2012

Fluorescent magnetic bead and cell differentiation/counting using a CMOS SPAD matrix

Edoardo Charbon, Martinus Gijs, Ulrike Lehmann, Caroline Vandevyver, Emile Dupont

We present a monolithic silicon chip comprising a matrix of 84 single photon avalanche diodes (SPADs) to detect and discriminate fluorescent beads or fluorescently labeled single cells in a polydimethyl(-siloxane) (PDMS) cartridge that is positioned on top ...

Elsevier2012

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

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Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

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