Structural and Functional Stochastic Super-Resolution Microscopy
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Microscopy is of high interest for biology since it allows imaging features that are too small to
be seen with naked eyes. However, cells are mostly transparent to visible and infrared light
which makes it difficult to see with a traditional microscope. To ...
Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...
In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...
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Point localization superresolution microscopy enables fluorescently tagged molecules to be imaged beyond the optical diffraction limit, reaching single molecule localization precisions down to a few nanometers. For small objects whose sizes are few times t ...
Super-resoln. imaging of living cells can reveal nanoscopic details of live biol. systems. The development of small-mol. fluorophores that allow optimal imaging conditions is the key to enable live-specimen imaging with minimal invasiveness. The authors re ...
Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...
Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...
We present a dielectric microsphere-based super resolution optical microscope that can image a sample beyond the classical diffraction limit [1]. The super resolution imaging capability of a single microsphere normally is restricted to an area of similar t ...
High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...