Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications
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The understanding of catalytic and enzymic processes at the mol. level is one of the most challenging problems in chem. and biol. A necessary prerequisite for any theor. modeling of these chem. reactions is an appropriate description of the electronic stru ...
A prototype kinetic model of bacterial glycolysis is formulated and a minimal set of assumptions is postulated that allows the formulation of an approx. math. model. To establish a baseline for future investigations of more complicated situations, it is as ...
The transfer of cyclodextrins to unusual acceptors in order to synthesize functionalized linear dextrins with the cyclodextrin glycosyltransferase from Klebsiella pneumoniae was investigated. This enzyme catalyzed the transfer of a-cyclodextrin only. The a ...
The pH-dependence of the reaction kinetics of lactase (b-galactosidase) [9031-11-2] from A. oryzae in different reaction media is presented in terms of a 2-parameter model. The lactase from A. oryzae seems to have replaced the A. niger lactase on the marke ...
The photoprocesses of N-vinylcarbazole (NVC) solubilized in the interior of an oil-in-water microemulsion (CTA-ci, hexadecyltrimethylammonium counter ion) functionalized by an oxidizing agent, S2O2−8, were studied using ruby laser and spectrofluorimeter te ...
The influence of temp. on the catalytic activity and deactivation of sol. enzymes which are com. used in batch processes is discussed. Since high temp. not only accelerates the desired enzymic reaction but also the undesired deactivation of the enzyme, it ...
Facilitated ammonium ion transfer reactions, using the crown ether dibenzo-18-crown-6, at a single microhole supported water/1,2-dichloroethane interface were used as the transducer to detect the biomolecule creatinine. The ammonium ion was produced during ...
The aim of the invention is to improve existing amperometric sensors used to measure the quantity of a component present in a solution. This aim is reached by using a sensor provided with a measuring electrode (20) having at least one current collector (37 ...
The reaction behavior of C. rugosa lipase in the presence of sol. substrates is reported, and conclusions are drawn relative to the enzymic mechanism. The expected interpretation in terms of the formation of a dimeric species of the enzyme has found a riva ...
Poly(A) polymerase was purified 22,000-fold to homogeneity from a whole cell extract of Saccharomyces cerevisiae with a yield of 22%. The enzyme is a monomeric polypeptide with a denatured molecular weight of 63,000. Incorporation of labeled ATP into acid- ...
