Publication
Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications
Publications associées (51)
Development of degenerate and specific PCR primers for the detection and isolation of known and putative chloroethene reductive dehalogenase genes
Christof Holliger, Julien Maillard
Degenerate and specific PCR primers were designed for the detection of chloroethene reductive dehalogenases (CE-RDase), the key enzymes of chloroethene dehalorespiration, based on sequence information of three CE-RDases and three chlorophenol (CP) RDases. ...
2004Reaction mechanism of caspases: Insights from QM/MM Car-Parrinello simulations
Caspases are fundamental targets for pharmaceutical interventions in a variety of diseases involving disregulated apoptosis. Here, we present a quantum mechanics/mol. mechanics Car-Parrinello study of key steps of the enzymic reaction for a representative ...
2003Protonation state of Asp120 in the binuclear active site of the metallo-beta-lactamase from Bacteroides fragilis
The determination of the protonation state of enzyme active sites may be crucial for the investigation of their mechanism of action. In the bizinc beta-lactamase family of enzymes, no consensus has been reached on the protonation state of a fully conserved ...
2003Examining reactivity and specificity of cytochrome c peroxidase by using combinatorial mutagenesis
Kai Johnsson, Claudio Arrivoli, Petra Tafelmeyer, Martin Wilming
Combinatorial mutagenesis was used to investigate the role of three key residues in cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae, Arg48, Trp51, and Trp191, in control of the reactivity and selectivity of the heme-contg. enzyme. Libraries wer ...
2002Drug resistance in HIV-1 protease: flexibility-assisted mechanism of compensatory mutations
Ursula Röthlisberger, Paolo Carloni
The emergence of drug-resistant variants is a serious side effect assocd. with acquired immune deficiency syndrome therapies based on inhibition of human immunodeficiency virus type 1 protease (HIV-1 PR). In these variants, compensatory mutations, usually ...
2002Two General Methods for the Isolation of Enzyme Activities by Colony Filter Screening
We describe two general methodologies, based on filter-sandwich assays, for isolating enzymic activities from a large repertoire of protein variants expressed in the cytoplasm of E. coli cells. The enzymes are released by the freezing and thawing of bacter ...
2002A short and flexible route to aza-beta-(1 -> 6)-C-disaccharides: Selective alpha-glycosidase inhibitors
The syntheses of azaMan-beta-(1-->6)-C-Glc (4), azaGlc-beta-(1-->6)-C-Glc (5), and azaGal-beta-(1-->6)-C-Glc (6) based upon double reductive amination of acetylenic carbohydrate-derived diketones is described. The required diketones are obtained by additio ...
1999Ab initio and hybrid molecular dynamics simulations of the active site of human carbonic anhydrase II: a test case study
Ab initio mol. dynamics (AIMD) and combined Hybrid/AIMD simulations appear as promising candidates for an in situ modeling of enzymic reactions. In order to probe the capabilities of these methods for the characterization of enzymic processes we have chose ...
1998Positional cloning of the zebrafish sauternes gene: A model for congenital sideroblastic anaemia
Many human anaemias are caused by defects in haemoglobin synthesis. The zebrafish mutant sauternes (sau) has a microcytic, hypochromic anaemia, suggesting that haemoglobin production is perturbed. During embryogenesis, sau mutants have delayed erythroid ma ...
1998The disulfide bond in the Aeromonas hydrophila lipase/acyltransferase stabilizes the structure but is not required for secretion or activity
Françoise Gisou van der Goot Grunberg
Vibrio and Aeromonas spp. secrete an unusual 35-kDa lipase that shares several properties with mammalian lecithin-cholesterol acyltransferase. The Aeromonas hydrophila lipase contains two cysteine residues that form an intramolecular disulfide bridge. Here ...
1997