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Double-helix enhanced axial localization in STED nanoscopy

Publications associées (32)

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Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

Contrast Enhancement in Optical Coherence Microscopy

Christophe Pache

Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...

EPFL2012

Beyond the lateral resolution limit by phase imaging

Christian Depeursinge, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a theory to extend the classical Abbe resolution limit by introducing a spatially varying phase into the illumination beam of a phase imaging system. It allows measuring lateral and axial distance differences between point sources to a higher ac ...

2011

Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy

Giorgio Margaritondo

Background: Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptak ...

BioMed Central2011

Hard x-ray Zernike microscopy reaches 30 nm resolution

Giorgio Margaritondo, Vincent Gajdosik

Since its invention in 1930, Zernike phase contrast has been a pillar in optical microscopy and more recently in x-ray microscopy, in particular for low-absorption-contrast biological specimens. We experimentally demonstrate that hard-x-ray Zernike microsc ...

Optical Society of America2011

Complex index refraction tomography with sub ?/6-resolution

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte

The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...

2011

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

Nicolas Pavillon

We present in this PhD thesis work various applications of digital holographic microscopy (DHM), an imaging technique based on coherent illumination which enables the recovery of the full complex wavefront, i.e. the amplitude and phase of a wave field whic ...

EPFL2011

Dark-field optical coherence microscopy

Theo Lasser, Christophe Pache, Martin Villiger

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Three-dimensional Nano-Localization of Fluroescent Emitters

Theo Lasser, Iwan Märki, François Aguet, Noelia Laura Bocchio

The use of fluorescence light for far-field imaging at the nanometer scale has drawn much attention worldwide. Example techniques include STED microscopy and localization microscopy (PALM, STORM). Typically, nanoscopy approaches have been focused on the ma ...

2009

The high brightness and coherence of X-ray synchrotron sources and steady development in X-ray optics are critical ingredients in novel imaging techniques for a variety of domains. We employed several different approaches and a wide energy spectrum of radi ...

EPFL2009