Publication
Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Publications associées (46)
Weak and Transient Protein Interactions Determined by Solid-State NMR
Despite their roles in controlling many cellular processes, weak and transient interactions between large structured macromolecules and disordered protein segments cannot currently be characterized at atomic resolution by Xray crystallography or solution N ...
Wiley-Blackwell2016Structure and Mechanism of the Influenza A M2(18-60) Dimer of Dimers
We report a magic angle spinning (MAS) NMR structure of the drug-resistant S31N mutation of M2(18-60) from Influenza A. The protein was dispersed in diphytanoyl-sn-glycero-3-phosphocholine lipid bilayers, and the spectra and an extensive set of constraints ...
American Chemical Society2015Nanosecond Time Scale Motions in Proteins Revealed by High-Resolution NMR Relaxometry
Geoffrey Bodenhausen, Fabien Ferrage
Understanding the molecular determinants underlying protein function requires the characterization of both structure and dynamics at atomic resolution. Nuclear relaxation rates allow a precise characterization of protein dynamics at the Larmor frequencies ...
American Chemical Society2013Method for nuclear magnetic resonance (NMR) spectroscopy measurements using long-lived coherences (LLC)
Geoffrey Bodenhausen, Paul Romeo Vasos, Riddhiman Sarkar, Puneet Ahuja
A method for nuclear magnetic resonance (NMR) spectroscopy of a sample comprises preparation of the sample and carrying out an NMR spectroscopy measurement. Preparation includes excitation of long lived coherences (LLC) between the singlet state S0 and the ...
2013Dynamic Studies through Control of Relaxation in NMR Spectroscopy
In numerous biological processes that constitute the base of living organisms, protein function is fundamentally related to internal dynamics occurring on μs-ms time scales that can give rise to chemical exchange contributions to relaxation. In a heteronuc ...
EPFL2013Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Geoffrey Bodenhausen, Diego Carnevale
We systematically studied the enhancement factor (per scan) and the sensitivity enhancement (per unit time) in C-13 and Si-29 cross-polarization magic angle spinning (CP-MAS) NMR boosted by dynamic nuclear polarization (DNP) of functionalized mesoporous si ...
Royal Society of Chemistry2013Structure and backbone dynamics of a microcrystalline metalloprotein by solid-state NMR
We introduce a new approach to improve structural and dynamical determination of large metalloproteins using solid-state nuclear magnetic resonance (NMR) with H-1 detection under ultrafast magic angle spinning (MAS). The approach is based on the rapid and ...
NATL ACAD SCIENCES2012Rapid Measurement of Pseudocontact Shifts in Metalloproteins by Proton-Detected Solid-State NMR Spectroscopy
Pseudocontact shifts (PCSs) arise in para-magnetic systems in which the susceptibility tensor is anisotropic. PCSs depend upon the distance from the paramagnetic center and the position relative to the susceptibility tensor, and they can be used as structu ...
AMER CHEMICAL SOC2012H-1, C-13 and N-15 resonance assignment of a 114-residue fragment of Engrailed 2 homeoprotein, a partially disordered protein
Geoffrey Bodenhausen, Fabien Ferrage
Engrailed 2 is a transcription factor belonging to the class of homeoproteins. These proteins possess a 60-residue DNA binding globular domain and play an important role in the early stages of development. We expressed and purified a 13.4 kDa fragment of E ...
2011Analysis of Cancer Metabolism by Imaging Hyperpolarized Nuclei: Prospects for Translation to Clinical Research
A major challenge in cancer biology is to monitor and understand cancer metabolism in vivo with the goal of improved diagnosis and perhaps therapy. Because of the complexity of biochemical pathways, tracer methods are required for detecting specific enzyme ...
2011