Publication

Determination of sub-diffraction-limited organelles and biomolecules using microsphere lenses in combination with fluorescence microscopy

Publications associées (83)

Optical microscopy using a glass microsphere for metrology of sub-wavelength nanostructures

A technique that allows direct optical imaging of nanostructures and determines quantitatively geometric nanofeatures beyond the classical diffraction limit by using high-refractive index glass microspheres is introduced. The glass microsphere is put on a ...

Elsevier2015

Digital confocal microscopy through a multimode fiber

Demetri Psaltis, Christophe Moser, Ioannis Papadopoulos, Damien Claude-Marie Loterie, Salma Farahi, Alexandre Goy

Confocal laser-scanning microscopy is a well-known optical imaging method where a pinhole is used in the illumination and detection pathways of a normal microscope, in order to selectively excite and detect a particular focal volume. The advantage of this ...

Spie-Int Soc Optical Engineering2015

Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

Michael Stefan Daniel Liebling

We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...

2015

Broadband Digital Holographic Camera for Microscopy

Zahra Monemhaghdoust

In this thesis, we explore a combination of analog and digital holographic techniques to demonstrate high speed quantitative phase imaging (QPI) using low coherence (broad bandwidth) light sources. Off-axis digital holographic microscopy is inherently a co ...

EPFL2015

Photonic Nanojet Array for Fast Detection of Single Nanoparticles in a Flow

Martinus Gijs, Matteo Cornaglia, Hui Yang

We detect by optical microscopy Au and fluorescent nanopartides (NPs) during their motion in water based medium, using an array of dielectric microspheres that are patterned in a microwell array template. The Microspheres act as lenses focusing the light o ...

American Chemical Society2015

Fast detection of single nanoparticles in a microfluidic channel by a microlens array in combination with a conventional optical microscope

Martinus Gijs, Matteo Cornaglia, Hui Yang

We present the use of a microlens array in combination with a conventional optical microscope set-up for the detection of single nanoparticles (NPs) in fluid medium. Optically transparent dielectric microspheres are patterned in a microfabricated well arra ...

2015

Single molecule fluorescence resonance energy transfer scanning near-field optical microscopy: potentials and challenges

Giovanni Dietler, Mounir Driss Mensi, Anton Smirnov, Kanat Dukenbayev, Ekaterina Rostova, Andrey Mikhaylov

A few years ago, single molecule Fluorescence Resonance Energy Transfer Scanning Near-Field Optical Microscope (FRET SNOM) images were demonstrated using CdSe semiconductor nanocrystal-dye molecules as donor-acceptor pairs. Corresponding experiments reveal ...

Royal Soc Chemistry2015

Super-resolution optical microscopy using a glass microsphere nanoscope

Martinus Gijs, Hui Yang

2014

Developing luminescent Brownian probes for near-field investigations of the intracellular environment

Flavio Maurizio Mor

Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...

EPFL2013

Structural and Functional Stochastic Super-Resolution Microscopy

Stefan Geissbühler

Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...

EPFL2013

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