Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization
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One microband of an array of four microband electrodes (1 mm long and 25 μm wide with a 25 μm gap) was modified with glucose oxidase by direct electrochemically assisted immobilisation, giving a stable microbiosensor with an apparent Michaelis-Menten const ...
In Saccharomyces cerevisiae, the genes HXK2 and REG1 are believed to play a central role in carbon catabolite repression, acting on multiple pathways in the central metab. The present study aimed at quantifying the effect of deletion of genes HXK2 or REG1, ...
Despite striking advances in functional brain imaging, the cellular and molecular mechanisms that underlie the signals detected by these techniques are still largely unknown. The basic physiological principle of functional imaging is represented by the tig ...
The syntheses of azaMan-beta-(1-->6)-C-Glc (4), azaGlc-beta-(1-->6)-C-Glc (5), and azaGal-beta-(1-->6)-C-Glc (6) based upon double reductive amination of acetylenic carbohydrate-derived diketones is described. The required diketones are obtained by additio ...
Glucose is the major substrate that sustains normal brain function. When the brain glucose concentration approaches zero, glucose transport across the blood-brain barrier becomes rate limiting for metabolism during, for example, increased metabolic activit ...
The amt. of Vitreoscilla Hb (VHb) expression was modulated over a broad range with an isopropyl-beta-D-thiogalactopyranoside- (IPTG)- inducible plasmid, and the consequences on microaerobic Escherichia coli physiol. were examd. in glucose fed-batch cultiva ...
We have investigated different possible mechanisms for the cis-trans isomerization in triply bonded ditungsten complexes with stoichiometry W2Cl4(NHEt)2(PMe3)2 using static d. functional calcns. as well as Car-Parrinello simulations. Our studies reveal an ...
The aim of the present work was to investigate whether uncoupling of catabolism from anabolism, which was often obsd. in heterotrophic microorganisms under energy-sufficient growth conditions, also occurs in the autotrophic bacterium M. thermoautotrophicum ...
Understanding the mechanism of brain glucose transport across the blood- brain barrier is of importance to understanding brain energy metabolism. The specific kinetics of glucose transport nave been generally described using standard Michaelis-Menten kinet ...
A tight coupling exists between neuronal activity and energy metabolism. Over a century ago Roy and Sherrington postulated that “the brain possesses intrinsic mechanisms by which its vascular supply can be varied locally in correspondence with local variat ...