Evaluation of pathogens inactivation during thermophilic anaerobic digestion of faecal sludge

There is an urgent need to develop comprehensive and sustainable sanitation systems in developing countries, including faecal sludge (FS) treatment technologies that help reduce environmental and human health risks. Anaerobic digestion (AD) is widely used for the treatment of biowastes but its potential for FS sanitization still needs to be assessed. The goal of this work was to evaluate pathogens inactivation during thermophilic AD of a synthetic surrogate for FS through biomethane potential (BMP) tests. Salmonella, Ascaris suum eggs and phages T4 and MS2 were selected as models for bacterial, parasitic and viral pathogens, respectively. Salmonella were not detected anymore after 110 minutes of thermophilic AD. In anaerobic conditions at 55°C, concentrations of phages T4 and MS2 were below detection levels after 3 and 4 days, and no potentially viable Ascaris eggs were found after 15 days. Methods need to be optimized in order to improve the reliability of viability checks on Ascaris eggs. Future experiments could focus on this model, which will a priori be the limiting factor for the dimensioning of anaerobic digesters that yield safe-to-use end products.

Faecal sludge treatment: Optimization of Ascaris eggs enumeration and inactivation, and start-up of an anaerobic thermophilic acid-phase digester

Léa Cécile Randin

Faecal sludge (FS) accumulates in onsite sanitation technologies, which play a prevailing role in low- and middle-income countries. However, FS management is generally lacking. FS is usually directly discharged in the environment despite its high organic load and concentration in pathogens, including parasites such as Ascaris sp.. It is therefore urgent to find an adequate treatment to stabilise and sanitize FS. One possibility, which still needs to be assessed, is the so called temperature-phased anaerobic digestion (TPAD). The main objective of this study was to evaluate the potential of inactivation of Ascaris eggs in the first phase of TPAD, the acid-phase. However, standard methods to enumerate Ascaris eggs – which are microscopic methods involving an incubation - are lacking. The first part of this study focused on the improvement of existing methods to reliably evaluate the treatment performances. Then, inactivation efficiency of Ascaris eggs at 55°C in pure solution and sludge was evaluated. Inactivation efficiency was finally tested after injection of Ascaris eggs in a 1L anaerobic reactor operated at 55°C, which had been started-up to favour the acid-phase. By following the development of the eggs during the incubation time, it was observed that a period of incubation of 14 days is appropriate. Indeed, 14 days allow all eggs to develop to the larval stage while reducing the loss of eggs due to hatching or lysis. It was also found that the presence of FS does not influence the time of incubation. The inactivation experiments have shown that the temperature as a clear impact on the eggs, and that inactivation was accelerated in the presence of substrate and by anaerobic conditions. Indeed, eggs were inactivated within 3 hours when they were heated at 55°C, in 1h30 when they were introduced in digested sludge at 55°C and in less than 45 minutes when they were injected in the acid phase reactor at 55°C. The exploitation of the acid-phase reactor, with synthetic FS imitating the characteristics of FS as a substrate, allows recommending a hydraulic retention time of four days. This enables to run a stable acid-phase process. Standard methods for the enumeration of Ascaris eggs propose an incubation time of three to four weeks. Its reduction to two weeks is a clear advantage as it decreases the time before obtaining the results. Moreover, this study has shown that TPAD is a possible technology to inactivate Ascaris eggs. If the second phase of TPAD permits to stabilise FS, then this technology would be an adequate treatment for FS in low- and middle-income countries.

2015

Vers un assainissement urbain durable en Afrique subsaharienne

Halidou Koanda

Justification In Sub-Saharan Africa, excreta and wastewater disposal is characterised primarily by on-site sanitation systems. This sanitation option is more likely to increase as governments, municipalities, communities, private operators and donor agencies strive to reach the Millennium Development Goals aimed at reducing by half the proportion of people without access to basic sanitation facilities. Due to increased urban population growth in Sub-Saharan Africa, the faecal sludge volumes will increase to alarming quantities in the coming decades. An evaluation of faecal sludge management practices reveals that the main shortcomings relate to the widespread lack of involvement and foresight by the municipalities, the paucity of effective legal provisions and organization, and the absence of stakeholder coordination. Stakeholder involvement for planning and implementing faecal sludge management is virtually inexistent or excluded from urban sanitation planning processes. In the majority of situations, faecal sludge management is financially not viable. This is a major cause of the indiscriminate dumping of faecal sludge in urban areas or its untreated use in urban agriculture. These practices not only pose permanent and serious health risks but also contaminate both scarce ground and surface water. Objective and general approach On the basis of these facts, which present a real challenge to sector specialists, the overall objective of our research is to develop an innovative strategic planning approach for sustainable faecal sludge management in urban areas which allows minimising public health and environmental risks. Focus is placed on developing a reproducible methodology for stakeholder involvement, on enhancing population awareness for improved faecal sludge disposal and attributing a leading role to the dynamic private faecal sludge emptying and transport operators. These aspects form the three pillars of our search for a comprehensive methodological approach. The municipality of Ouahigouya in Burkina Faso with a population of about 65'000 inhabitants was used as pilot site to launch, implement and scientifically validate the approach. Analysis and stakeholder involvement The stakeholder-based planning approach developed in Ouahigouya relies on a combination of stakeholder identification and analysis, and participatory planning techniques (focus groups, workshops, informal meetings, all-stakeholder workshops). A quantitative survey among households allowed collecting stakeholders' proposals and evaluating suggestions for improvement of faecal sludge management and neighbourhood hygiene proposed by the population. The main steps in the stakeholder involvement approach include: Jointly clarifying the objectives of the planning process together with the stakeholders; Identifying stakeholders and their interests; Classifying stakeholders according to their importance and influence based on defined criteria and confirmed by the stakeholders themselves; Identifying the interactions between the stakeholders; Verifying the results of the stakeholder analysis by the stakeholders themselves; Choosing stakeholder involvement techniques; Implementing the chosen method of stakeholder involvement: development of faecal sludge management scenarios, acceptance of the scenarios by the stakeholders in focus groups, choice and acceptance of a basic scenario for development of the future strategy in an all-stakeholder workshop; Evaluating the chosen involvement process by the stakeholders. The experience gained with the planning methods developed and implemented in Ouahigouya reveals that the "Stakeholder analysis" method is efficient and provides answers to questions such as: Who are the important and influential stakeholders? Who should participate in the planning process and in which manner? This experience also revealed the need for capacity building of certain stakeholder categories (emptying operators, women's associations, etc.) to increase their degree of involvement in the planning process. Improvement in stakeholder involvement was achieved thanks to a combination of workshops, focus groups and other informal meetings allowing less influential stakeholders (associations of local women, horticulturists, emptiers) to share information and to freely express their opinions and concern and to defend their interests. Thus, providing an excellent example of local democracy appreciated by all stakeholders of Ouahigouya. The approach developed provides tools for policy-makers and planners to ensure an effective stakeholder involvement in the process of developing a common vision and implementation of improved urban sanitation measures. The conditions for replicating the approach developed and tested in Ouahigouya are due to participatory democracy, great commitment of the local authorities and the high motivation of the stakeholders involved. Levers for the willingness-to-improve faecal sludge management and neighbourhood hygiene The evaluation method of the willingness to improve faecal sludge management by the population was based on a combination of the planned behavior model of Ajzen and the contingent valuation method, financial analysis of the pit emptying and faecal sludge enterprise, and on focus groups of stakeholders. By focusing on local practices, perceptions and individual behavior, this method revealed that financial viability – often reduced to willingness-to-pay – is not the only factor to consider when planning improvement of sanitation practices. Willingness-to-improve is highly dependant on psycho-sociological factors such as attitude towards and beliefs in an improved neighbourhood environment, social pressure on the households by the neighbourhood as well as the subjective costs and benefits expected from improved faecal sludge management. These factors therefore present levers allowing planners and decision-makers to encourage, increase and develop the demand for improved latrine emptying services, e.g. though social marketing. Promoting private entrepreneurs According to market analysis of emptying services and stakeholders involved, small private and mainly informal enterprises play a leading role in faecal sludge management in most cities of Sub-Saharan Africa. These operators, dynamic but fragile, require an appropriate institutional, jurisdictional and economic environment based on a balanced partnership between the municipality (public) and the private sector. Our study reveals that for a company truck to be profitable, about 1'000 trips per year (at 6'000 FCFA per trip) are required or the equivalent of a city of 56'000 inhabitants. In the case of Ouahigouya, profitability is increased if the emptying operators apply the latest management and professional tools and techniques, and manage to provide emptying services in neighbouring towns. Decision support tools The research led to developing decision support tools for professionals and policy-makers. These tools facilitate the development of a tariff-oriented policy to avoid uncontrolled faecal sludge discharge into the environment, to render investments by private operators profitable, and still make emptying services accessible to low-income households. This involves: Analysis of the cost structure of 3 pit emptying companies has revealed that fuel, maintenance/repair and personnel costs make up 45%, 20% and 30%, respectively, of an enterprise's operating expenses. An Excel programme was developed to allow municipalities and private operators to determine the operating costs of emptying services. This allowed to establish a sustainable money flux between the different stakeholders involved and to define the tariffs. To prevent faecal sludge discharges in the immediate residential environment or on unauthorised sites, it might under specific circumstances prove necessary and feasible to reimburse rather than charge entrepreneurs for delivering faecal sludge to the treatment sites, hence, create a strong incentive for good emptying and haulage practice. Evaluation methods of the sludge quantities to be disposed of have been developed to allow faecal sludge planning by the municipal authorities, assessment of the potential market and return on investment by the private operators. Advantages of the approach for the population of Ouahigouya Based on the results of our study, it was possible to formulate practical recommendations for the organisation of the urban sanitation sector for the municipality of Ouahigouya – now in possession of a sustainable faecal sludge management strategy. Our study has also contributed to promoting local consultation and raising the awareness of the role and contribution of each stakeholder group, in particular the previously neglected manual emptiers in their daily efforts to improve the living conditions of the urban inhabitants. The "Strategic Excreta and Wastewater Sanitation Plan", that was developed and supported by the "Office National de l'Eau et de l'Assainissement - National Water and Sanitation Agency", provides an excellent framework for the implementation of the elaborated faecal sludge management model (as part of the this strategic plan).

EPFL2006

Identification of parameters affecting the outcome of biochemical methane potential (BMP) tests

Jonas Stöckli

Anaerobic digestion of organic waste to methane gas (CH4) is an attractive method to produce renewable energy. Due to the trend towards the reuse of waste and away from fossil energy sources, this technology saw a worldwide development in the recent years. A fundamental parameter in this domain is the Biochemical Methane Potential (BMP), which defines the amount of CH4 which can be produced out of a certain organic substrate. Such information is essential in order to plan and optimise anaerobic digestion plants, and to evaluate the reactor feed with a new substrate or a new substrate combination (co-digestion). BMPs are assessed in batch test, which can be regarded as the simulation of a full-scale biogas plant. Two experiments are required, the test in which the test substrate is digested on the inoculum, and the blank, in which the inoculum is digested alone. The part of the inoculum which can be digested is called endogenous substrate. Since the test experiment produced not only on the test substrate but only on the endogenous substrate, the CH4 production on the test substrate alone is obtained by the subtraction of the test production minus the blank production. The units of a BMP are generally indicated in litre of CH4 per gram of volatile solids (VS) test substrate [L/gVS]. Despite the importance and the wide application of this parameter, its exact determination remains a challenging issue and results are often not consistent in between (inter-) and within (intra-) laboratories. Experimental protocols exist but do not lead to a satisfactory BMP tests consistency neither. The aim of this study was to identify parameters affecting the outcome of BMP tests, based on the investigation of two different data sets. An inter-laboratory study of BMP tests providing the final BMP values of 327 experiments and information about 40 related experimental parameters, and a second data set containing the complete CH4 production curves of 136 BMP experiments provided by one single laboratory. The method consisted in graphical and statistical analysis (Mixed Effect Modelling), using [R] programming language and software environment. This study found out, that a significant part of the inter-laboratory BMP inconsistency can be explained by an imprecise assessment of the VS, which was not expected. As the VS of the substrate are directly implied in the computation of the BMP, the impact was significant and therefore the BMP were corrected regarding the VS imprecision. With these data, up to 70% of the inconsistency of BMPs could be explained by inter-laboratory effects. The statistical analysis led to the conclusion that the concentration of the endogenous substrate and the moisture content in the digestate would be the principal factors affecting the outcome of BMP tests. An effort was made to identify and correct errors contained in CH4 production curves, which turned out to be delicate for certain cases. Also indications regarding the precision and the reliability of BMPs were formulated. Further, the investigation of the CH4 production curves led to the development of a new method in order to compute the BMP result. This method was based on the fact, that a certain inoculum reaches always the same slope toward the end of the experiment, no matter what was digested before. The advantage of this method would be that the experiment end-point could be set clearly and that the concentration of the endogenous substrate would not have an impact on the outcome of a BMP test. According to the findings of this report, it was proposed to add the following requirements into experimental protocols for BMP tests: Tests, blanks and the analysis of VS should be carried out in triplicates and their standard deviation should be indicated for each of them, together with the BMP result If triplicates contain set-up errors, these experiments must be repeated, including the corresponding blank/test In the annex: An experimental protocol for the TS and VS analyses An indication of a required moisture content of the digestate (mechanism to investigate in detail first) An indication of the range of required VS concentrations or a maximal production rate for blank tests according to certain experimental conditions (mechanism to investigate in detail first). This report identified several parameters, which contribute to the inter-laboratory inconsistency of BMPs. These findings should be investigated further in order to prove and quantify their impact. An effort should be made to demonstrate the newly developed BMP-computation-method, which could eventually lead to more consistent results. The limitation of this study was, that a relatively low amount of data was available compared to their characteristics. Consequently, the findings could only be proven on a few examples and should therefore only be seen as evidences. Further, this led also to the risk of overfitting, since a relative high amount of parameters needed to be included into the statistical model.

2012