Publication

STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

Publications associées (36)

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We propose the use of a high-refractive index glass microsphere in combination with a conventional fluorescence microscope for imaging sub-cellular organelles and biomolecules. The microsphere is placed on a sample that is immersed in water, collects the n ...

Spie-Int Soc Optical Engineering2015

Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation

Tomas Lukes

We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluo ...

Optical Society of America2014

Optical microscopy for biology and life sciences

Theo Lasser, Marcel Leutenegger

Optical microscopy has become a major non- or minimally invasive tool for investigating living organisms such as cells, tissues or small animals. Being such an essential tool for research and because lacking knowledge and insight in the working principles ...

Laboratoire d'Optique Biomédicale, EPFL2014

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Suliana Manley, Luc Reymond, Grazvydas Lukinavicius, Aleksandr Benke, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Pierre Gönczy, Suliana Manley, Romain Wyss, Debora Keller

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller Univ Press2014

Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...

EPFL2013

Functional and Structural Imaging with Optical Coherence Microscopy

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

The aim of the present PhD thesis is the elaboration of new, home-made Scanning Near-field Optical Microscope (SNOM or NSOM), and the demonstration of its great potential for high-resolution topography and fluorescence investigations of soft and solid samp ...

EPFL2011

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A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...

2010

Biopolymer-encapsulated single-walled carbon nanotubes (SWNT) recognize specific nitroarom. mols. via either a wavelength or intensity change of near IR photoluminescence. A class of peptides from the bombolitin family not previously identified for nitroar ...

American Chemical Society2010