Tension-induced fusion of bilayer membranes and vesicles
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The smaller isoform of the GABA synthesizing enzyme glutamic acid decarboxylase, GAD65, is synthesized as a soluble protein that undergoes posttranslational modification(s) in the NH2-terminal region to become anchored to the membrane of small synaptic-lik ...
For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...
Purified mitochondrial creatine kinase (Mi-CK) (EC 2.7.3.2) from chicken heart was shown to interact simultaneously with purified inner and outer mitochondrial membranes, thereby creating an intermembrane chondrial membranes, thereby creating an intermembr ...
American Society for Biochemistry and Molecular Biology1991
In the rabbit nephron, the luminal membrane surface area of the proximal convoluted tubule (PCT) is more than twice that of the proximal straight tubule (PST). What seemed to be an increase in histological specialization in solute and water transport is cu ...
Colicins A and N are pore-forming bacterial toxins that kill Escherichia coli cells. Their mode of action involves three steps; binding to specific receptors located in the outer membrane, translocation through this membrane and the periplasm, and channel ...
Insertion of some protein toxins into membranes proceeds through an unfolding step. The unfolding trigger can be the low pH in endosomes, exposure to body temperature, reduction of disulphide bonds or proteolytic cleavage occurring at the membrane surface. ...
The mechanisms of water transport across the rabbit renal proximal convoluted tubule were approached by measuring osmotic permeabilities and solute reflection coefficients of the brush-border and the basolateral membranes. Plasma and intracellular membrane ...
A symposium report. The total synthesis of melittin-TASP, T4-(4a26) (TASP = template assembled synthetic proteins) is described together with its structural and ion-channel forming properties in planar lipid bilayers. A voltage-dependent channel activity i ...
A procedure is described for the isolation of sub-cellular fractions from bloodstream forms of Trypanosoma brucei. The method leaves intact most of the nuclei, mitochondria and microbodies. All the fractions have been chemically characterized and tested fo ...
CD and fluorescence were used to study the interaction of the model peptide melittin with dimyristoylphosphatidylcholine (DMPC) bilayer liposomes. Melittin binds tightly to the lipid membrane and undergoes a conformational change from a random to an .apprx ...
