Publication

Multi-plane super-resolution microscopy

Publications associées (160)

A PSF-Based Approach to Biplane Calibration in 3D Super-Resolution Microscopy

Michaël Unser, Daniel Sage, Hagay Kirshner

Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimensio ...

IEEE2012

Harmonic Nanocrystals for Bio-Labeling: a Survey of Optical Properties and Biocompatibility

Sandrine Gerber, Solène Marcelle Françoise Marie Passemard, Jean-Pierre Wolf, Jérôme Extermann, Luigi Bonacina, Daniel Rytz, Rachid Hadji

Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multi-photon microscopy. We present for the first time a complete survey of the properties of the different nanomaterials proposed to date, de ...

American Chemical Society2012

Supervoxel-Based Segmentation of Mitochondria in EM Image Stacks with Learned Shape Features

Pascal Fua, Graham Knott, Radhakrishna Achanta, Kevin Smith, Aurélien Lucchi

It is becoming increasingly clear that mitochondria play an important role in neural function. Recent studies show mitochondrial morphology to be crucial to cellular physiology and synaptic function and a link between mitochondrial defects and neuro-degene ...

Institute of Electrical and Electronics Engineers2012

Single Molecule Sensing

Claudio Dellagiacoma

Living organisms consist of cells, the elementary components of which are proteins providing cellular structures and functionality. Nowadays, proteins of the size of one to a few tens of nanometers can be efficiently monitored by fluorescence microscopy an ...

EPFL2011

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Marcel Leutenegger

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is maki ...

2011

Comparison between SOFI and STORM

Theo Lasser, Stefan Geissbühler, Claudio Dellagiacoma

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time usi ...

2011

Hybrid Small Animal Imaging System Combining Magnetic Resonance Imaging with Fluorescence Tomography Using Single Photon Avalanche Diode Detectors

Edoardo Charbon, Lucio Carrara, Markus Rudin, Christof Baltes

The high sensitivity of fluorescence imaging enables the detection of molecular processes in living organisms. However, diffuse light propagation in tissue prevents accurate recovery of tomographic information on fluorophore distribution for structures emb ...

Institute of Electrical and Electronics Engineers2011

Motion Parallax Based Restitution of 3D Images on Legacy Consumer Mobile Devices

Touradj Ebrahimi, Martin Rerabek, Jong Seok Lee, Lutz Goldmann

While 3D display technologies are already widely available for cinema and home or corporate use, only a few portable devices currently feature 3D display capabilities. Moreover, the large majority of 3D display solutions rely on binocular perception. In th ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2011

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

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