Phenotypic Heterogeneity in Mycobacterium tuberculosis
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We present a 3-D microfluidic device designed for localized drug delivery to cellular networks. The device features a flow cell comprising a main channel for nutrient delivery as well as multiple channels for drug delivery. This device is one key component ...
The analysis of circulating tumor cells (CTCs) from the blood of cancer patients promises a better understanding of metastasis formation and tumor phenotypes. CTCs are rare (there is 1 CTC in 1 billion of circulating blood cells) and therefore challenging ...
Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted ele ...
We present a novel optofluidic differential method for carrying out absorbance spectroscopy of subnanolitre volumes of liquid samples on a microfluidic chip. Due to the reduction of liquid volume, the absorbance detection in microfluidics is often hindered ...
The new antitubercular drug candidate 2-[2-S-methyl-1,4-dioxa-8-azaspiro[4.5]dec-8-yl]-8-nitro-6-(trifluoromethyl)-4H-1,3-benzothiazin-4-one (BTZ043) targets the DprE1 (Rv3790) subunit of the enzyme decaprenylphosphoryl-beta-d-ribose 2'-epimerase. To monit ...
Since the introduction of the of Micro Total Analysis Systems concept in 1990, important progress in microfluidic analytical systems has been realized. In many cases, microfluidic systems have shown improved analytical performances over classical methods, ...
This thesis reports on the integration of continuous-flow cell separation method for lab-on-a-chip applications. Cell separation methods are widely used in biology to prepare samples prior to analysis. There is a need for a highly sensitive separation meth ...
The main aim of this project was to develop novel concepts for miniaturization of bioanalytical techniques for investigating biomolecular interactions. We used optical tweezers to selectively address individual biological objects in microfluidic channels. ...
This article presents a new integrated microfluidic/microoptic device designed for basic biochemical analysis. The microfluidic network is Wet-etched in a Borofloat 33 (Pyrex) glass wafer and sealed by means of a second wafer. Unlike other similar microflu ...
versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to gener ...