Publication

Nanocapillaries combined with optical tweezers as a single molecule technique for studying DNA-protein complexes

Publications associées (85)

The double life of UPF1 in RNA and DNA stability pathways

The DNA and RNA helicase UPF1 is well known for its central role in Nonsense Mediated RNA Decay (NMD), which promotes degradation of mRNAs containing premature stop codons. However, we have recently demonstrated that human UPF1 is also essential for DNA re ...

2006

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

Hydrogenated amorphous silicon ultraviolet sensor for deoxyribonucleic acid analysis

Giovanni De Cesare, Carlotta Guiducci

In this letter, we show the results achieved using a hydrogenated amorphous silicon photosensor for a "label-free" deoxyribonucleic acid (DNA) analysis based on the measurements of the absorbance in the ultraviolet range. The optimization of the sensor str ...

2006

Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts

Philipp Bucher

The function of DNA-binding proteins is controlled not just by their abundance, but mainly at the level of their activity in terms of their interactions with DNA and protein targets. Moreover, the affinity of such transcription factors to their target sequ ...

2005

Supramolecular Nanostamping: Using DNA as Movable Type

Francesco Stellacci

Here we present a novel printing technique (that we call supramolecular nanostamping), based on the replication of single-stranded DNA features through a hybridization-contact-dehybridization cycle. On a surface containing features each made of single-stra ...

2005

Binding of organoruthenium anticancer drugs to DNA

Ursula Röthlisberger, Ivano Tavernelli, Christian Gossens

Organoruthenium compds. have emerged as promising novel anticancer agents but very little is known about their mol. mechanism of action. Here, we present classical and mixed QM/MM first-principles MD simulations of two ruthenium(II) compds. and their bindi ...

2005

Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities

Christof Holliger, Julien Maillard, Katia Szynalski

We developed standard curves based on plasmids containing a 16S rRNA gene of a member of one of the three genera Dehalobacter, Desulfitobacterium, and Dehalococcoides. A large difference in amplification efficiency between the standard curves was observed ...

2004

A general method for the covalent labeling of fusion proteins with small molecules in vivo

Horst Vogel, Kai Johnsson, Horst Pick, Thomas Gronemeyer, Antje Keppler

Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...

2003

Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Jürgen Brugger

A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of ...

2003

A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins

Philipp Bucher

Computer analysis of a conserved domain, BRCT, first described at the carboxyl terminus of the breast cancer protein BRCA1, a p53 binding protein (53BP1), and the yeast cell cycle checkpoint protein RAD9 revealed a large superfamily of domains that occur p ...

1997

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