A Microfluidic Platform Enables Large-Scale Single-Cell Screening to Identify Genes Involved in Bacterial Persistence
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A new population model is proposed for the growth of Saccharomyces cerevisiae and thereafter identified during synchronized continuous cultures where the initiation of cell division can be easily monitored from online measurements. The new model predicts t ...
Among the molecular mechanisms that control the cell division cycle, proteolysis has emerged as a key regulatory process enabling cells to pass critical check points. Such proteolysis involves a cascade of enzymes including a multisubunit complex termed 26 ...
American Society for Biochemistry and Molecular Biology1995
An extensive random mutagenesis study on the function of nine residues in the subunit binding site of FimC was conducted to evaluate the mol. mechanisms of subunit binding by FimC and the contribution of chaperone surface residues. Five plasmid libraries o ...
Deutsche Akademie der Naturforscher Leopoldina2001
Max-Planck Institute, Hans-Knoll-Str. 8, D-07745 Jena, Germany. The cytotoxicity of several saturated and unsaturated marine diatom-derived aldehydes and an oxo-acid have been screened in vitro and in vivo against different organisms, such as bacteria, alg ...
Described is method for introducing a desired nucleic acid into a eukaryotic host cell, said method comprising a) admixing the desired nucleic acid, a mono- and/or divalent metal cation and a eukaryotic host cell lacking a cell wallb) incubating the mixtur ...
The interaction of a microbial pathogen with its host is a highly dynamic process shaped by evolution. Understanding cellular responses to microbial products is essential for a complete understanding of virulence. Similarly, studies aimed at determining ho ...
A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl gro ...
Biofouling is defined by the non-specific attachment of biological material (proteins, carbohydrates, prokaryotic cells and higher organisms) to surfaces upon their exposure to any biological fluid. Biofouling is a serious problem in many areas ranging fro ...
