Publication
Cell-free reconstitution reveals centriole cartwheel assembly mechanisms
Publications associées (36)
Protocols for Subtomogram Averaging of Membrane Proteins in the Dynamo Software Package
Cryo-electron tomography allows low-resolution three-dimensional (3D) viewing of cellular organelles and macromolecular complexes present as multiple copies within a tomogram. These structures are computationally extracted and averaged in order to obtain h ...
Frontiers Media SA2018Multicolor single-particle reconstruction of protein complexes
Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...
2018High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6
Pierre Gönczy, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle, Santiago Harald Andany
The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...
2018Centriole Biogenesis: From Identifying the Characters to Understanding the Plot
Pierre Gönczy, Niccolo Banterle
The centriole is a beautiful microtubule-based organelle that is critical for the proper execution of many fundamental cellular processes, including polarity, motility, and division. Centriole biogenesis, the making of this miniature architectural wonder, ...
Annual Reviews2017Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis
Pierre Gönczy, Romain Hamelin, Florence Armand, Coralie Busso, Paul Guichard, Virginie Hamel, Isabelle Fluckiger
Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and centrosomes. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguis ...
2017Basal body structure in Trichonympha
Trichonympha is a symbiotic flagellate of many species of termites and of the wood-feeding cockroach. Remarkably, this unicellular organism harbors up to over ten thousand flagella on its surface, which serve to propel it through the viscous environment of ...
Springer Nature2016Fluorescent Nanodiamond–Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging
Theo Lasser, Weina Liu, Miguel Sison
There is a continuous demand for imaging probes offering excellent performance in various microscopy techniques for comprehensive investigations. of cellular processes by more than, one technique. FluoreScent nanodianiond gold nanoparticles (END Au) consti ...
Amer Chemical Soc2016Chemical Genetic Screen Identifies Natural Products that Modulate Centriole Number
Kai Johnsson, Pierre Gönczy, Michele Graciotti, Zhou Fang
Centrioles are microtubule-based organelles found in most eukaryotic cells and that are critical for the formation of cilia and flagella, as well as of centrosomes in animal cells. The number of centrioles must be strictly regulated in proliferating cells ...
Wiley-Blackwell2016Purification of centrosomes from mammalian cell lines
Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...
Elsevier Academic Press Inc2015The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation
Pierre Gönczy, Georgios Hatzopoulos, Nicola Jane Dynes, Sheng Kai Pong
Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evol ...
2015