Publications associées à Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest

Lighting up cells with lanthanide self-assembled helicates

Lanthanide bioprobes and bioconjugates are ideal luminescent stains in view of their low propensity to photobleaching, sharp emission lines and long excited state lifetimes permitting time-resolved detection for enhanced sensitivity. We show here how the i ...

Royal Society, The2013

Sorting Live Stem Cells Based on Sox2 mRNA Expression

Matthias Lütolf, Peter Frey, Jeffrey Alan Hubbell, Hans Mattias Larsson, Seung Tae Lee

While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary i ...

Public Library of Science2012

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Alessandra Griffa, Olivier Burri, Arne Seitz, Stefan Kobel, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012

The RADO approach to Quality-Driven Service Composition - Approximating the Pareto-Frontier in Polynomial Time

Boi Faltings, Immanuel Trummer

In Quality-Driven Service Composition, tasks from an ab- stract work ow are assigned to concrete services such that work ow QoS are optimized. The following three proper- ties are desirable for a corresponding algorithm. First, the run time is ideally boun ...

2012

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Philippe Renaud, Freddy Radtke, Rajwinder Lehal, Rodrigo Martinez Duarte

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of ...

2012

Effect of fluid flow shear stress on Mesenchymal Stem Cells(MSC) differentiation using a microfluidic device

Sohrab Emami Naeini

Mesenchymal Stem Cells (MSC) are multipotent stem cells that are predominantly obtained from the bone marrow. These cells have a high ability to differentiate into a multitude of cell types, which are very attractive for tissue engineering and cell therapy ...

2011

Optimal-Time Adaptive Strong Renaming, with Applications to Counting

Seth Gilbert, Dan Alistarh, Morteza Zadimoghaddam

We give two new randomized algorithms for tight renaming, both of which work against an adaptive adversary. The first uses repeated sampling over a sequence of arrays of decreasing size to assign unique names to each of

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processes with step complexity $ ...

Acm Order Department, P O Box 64145, Baltimore, Md 21264 Usa2011

Low Temperature Indium-based Sealing of Microfabricated Alkali Cells for Chip Scale Atomic Clocks

Nico de Rooij, Yves Pétremand, Rahel Strässle

In this paper, the development of a low temperature bonding process focused on indium-based technology for microfabricated alkali cells is presented. The intended application is the use of these cells in chip scale atomic clocks. The existing technology is ...

2010

Cell type dependence of carbon based nanomaterial toxicity

László Forró, Arnaud Magrez, Lenke Horváth, Beat Schwaller

Recent advances in nanotechnology led to the development of various nanostructures especially that of carbon-based nanomaterials (CBNs) for industrial and biomedical uses. As a result, the production as well as the human exposure to these materials will in ...

2010

Sorting signed permutations by inversions in O(nlogn) time

Bernard Moret, Yu Lin, Vaibhav Rajan, Krister Swenson

The study of genomic inversions (or reversals) has been a mainstay of computational genomics for nearly 20 years. After the initial breakthrough of Hannenhalli and Pevzner, who gave the first polynomial-time algorithm for sorting signed permutations by inv ...

2010

Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest

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Lighting up cells with lanthanide self-assembled helicates

Sorting Live Stem Cells Based on Sox2 mRNA Expression

Automated analysis of single stem cells in microfluidic traps

The RADO approach to Quality-Driven Service Composition - Approximating the Pareto-Frontier in Polynomial Time

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Effect of fluid flow shear stress on Mesenchymal Stem Cells(MSC) differentiation using a microfluidic device

Optimal-Time Adaptive Strong Renaming, with Applications to Counting

Low Temperature Indium-based Sealing of Microfabricated Alkali Cells for Chip Scale Atomic Clocks

Cell type dependence of carbon based nanomaterial toxicity

Sorting signed permutations by inversions in O(nlogn) time

Sorting Live Stem Cells Based on Sox2 mRNA Expression

Automated analysis of single stem cells in microfluidic traps

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Lighting up cells with lanthanide self-assembled helicates

The RADO approach to Quality-Driven Service Composition - Approximating the Pareto-Frontier in Polynomial Time

Optimal-Time Adaptive Strong Renaming, with Applications to Counting

Effect of fluid flow shear stress on Mesenchymal Stem Cells(MSC) differentiation using a microfluidic device

Low Temperature Indium-based Sealing of Microfabricated Alkali Cells for Chip Scale Atomic Clocks

Cell type dependence of carbon based nanomaterial toxicity

Sorting signed permutations by inversions in O(nlogn) time