Publication

Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells

Publications associées (49)

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Romain Guiet, Claudia Battistella

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

2019

In vitro fate mapping of stem cell development at the single cell level

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Hematopoietic stem cells (HSCs) are responsible for the continuous production of all blood cells. This unique ability has made it possible to successfully use HSCs in the clinical setting to remedy various blood disorders. However, despite six decades of r ...

EPFL2018

The core of this thesis is the application of different modalities of atomic force microscopy to study living systems. After a brief introduction, quantitative imaging state of the art is presented and the applications for mammalian, plant, bacterial, and ...

EPFL2018

High-speed atomic force microscopy (HS-AFM) is a scanning probe technique capable of recording processes at the nanometre scale in real time. By sequentially increasing the speed of individual microscope components, images of surfaces can be recorded at up ...

EPFL2018

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016

Perivascular Macrophages Limit Permeability

Michele De Palma, Melody Swartz, Mario Leonardo Squadrito, Witold Waldemar Kilarski, Esra Güç

Objective-Perivascular cells, including pericytes, macrophages, smooth muscle cells, and other specialized cell types, like podocytes, participate in various aspects of vascular function. However, aside from the well-established roles of smooth muscle cell ...

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Histology Staining Protocol Adaptation and Blood Smear Analysis

Iris Rentsch

Once a bone substitute, previously seeded with human bone progenitor cells, is implanted in a femoral condyle of a rat, it’s of importance to determine the intensity of a cell-triggered immune response of the host. Different techniques can be used, such as ...

2016

Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions

Esther Amstad, Gianluca Nicolas Etienne, Armend Gazmeno Hati

Understanding of cells has mainly been achieved through analysis of average properties of large cellular populations. The increased awareness of cellular heterogeneity in various biological systems, spans from single bacterial cells to human tissue calls f ...

RSC Publishing2016

Electrochemical push-pull probe: from scanning electrochemical microscopy (SECM) to multimodal altering of cell microenvironment

Hubert Girault, Andreas Stephan Lesch, Fernando Cortes Salazar, Dmitry Momotenko, Alexandra Bondarenko

To understand biological processes at the cellular level, a general approach is to alter the cells’ environment and to study their chemical responses. Herein, we present the implementation of an electrochemical push-pull probe, which combines a microfluidi ...

Amer Chemical Soc2015

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