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Infection by the Gram-negative bacterial pathogen Shigella flexneri depends on its ability to invade host cells. Bacterial engulfment requires a functional type III secretion system (TTSS) allowing the translocation into host cells of bacterial effectors t ...
The specific and covalent labeling of fusion proteins with synthetic mols. opens up new ways to study protein function in the living cell. Here the authors present a novel method that allows for the specific and exclusive extracellular labeling of proteins ...
Proteins exist in one of two generally incompatible states: either membrane associated or soluble. Pore-forming proteins are exceptional because they are synthesized as a water-soluble molecule but end up being located in the membrane -- that is, they are ...
We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier ...
The present invention relates to methods of transferring a label from novel substrates to O-alkylguanine-DNA alkyltransferases (AGT) and O-alkylguanine-DNA alkyltransferase fusion proteins, and to novel substrates suitable in such methods. Proteins o ...
A method for labeling acyl carrier protein (ACP) fusion proteins with a wide variety of different labels is disclosed. The method relies on the transfer of a label from a coenzyme A type substrate to an ACP fusion protein using a holo-acyl carrier protein ...
O6-substituted guanine derivs. are described for use as substrates for O6-alkylguanine-DNA alkyltransferases (AGT) that can be used to label the enzyme or fusion proteins contg. it. Proteins of interest are incorporated into an AGT fusion protein, the AGT ...
(Ecole Polytechnique Federale de Lausanne (EPFL), Switz.).2004
The outer membrane protein OmpF from Escherichia coli is a member of a large family of b-barrel membrane proteins. Some, like OmpF, are pore-forming proteins while others are active transporters or enzymes. We have previously shown that the receptor-bindin ...
The in vivo and in vitro labeling of fusion proteins with synthetic mols. capable of probing and controlling protein function has the potential to become an important method in functional genomics and proteomics. We have recently introduced an approach for ...
The structure of aperiodic crystals which includes incommensurate, quasi- and composite crystals is usually described in spaces of higher dimension, the so called superspace. The main advantage of the superspace formalism is that an aperiodic structure in ...
