Waveguide-PAINT offers an open platform for large field-of-view super-resolution imaging

Super-resolution microscopies based on the localization of single molecules have been widely adopted due to their demonstrated performance and their accessibility resulting from open software and simple hardware. The PAINT method for localization microscopy offers improved resolution over photoswitching methods, since it is less prone to sparse sampling of structures and provides higher localization precision. Here, we show that waveguides enable increased throughput and data quality for PAINT, by generating a highly uniform similar to 100 x 2000 mu m(2) area evanescent field for TIRF illumination. To achieve this, we designed and fabricated waveguides optimized for efficient light coupling and propagation, incorporating a carefully engineered input facet and taper. We also developed a stable, low-cost microscope and 3D-printable waveguide chip holder for easy alignment and imaging. We demonstrate the capabilities of our open platform by using DNA-PAINT to image multiple whole cells or hundreds of origami structures in a single field of view.

Anna Archetti, Evgenii Glushkov, Suliana Manley, Aleksandra Radenovic, Christian Sieben, Anton Stroganov

Super-resolution microscopies based on the localization of single molecules have been widely adopted due to their demonstrated performance and their accessibility resulting from open software and simple hardware. The PAINT method for localization microscopy offers improved resolution over photoswitching methods, since it is less prone to sparse sampling of structures and provides higher localization precision. Here, we show that waveguides enable increased throughput and data quality for PAINT, by generating a highly uniform ~100 × 2000 µm2 area evanescent field for TIRF illumination. To achieve this, we designed and fabricated waveguides optimized for efficient light coupling and propagation, incorporating a carefully engineered input facet and taper. We also developed a stable, low-cost microscope and 3D-printable waveguide chip holder for easy alignment and imaging. We demonstrate the capabilities of our open platform by using DNA-PAINT to image multiple whole cells or hundreds of origami structures in a single field of view.

Accordabilité de la réponse optique des cristaux photoniques par infiltration de matériaux organiques

Pascale El-Kallassi

Photonic crystals are stuctures in which the refractive index varies periodically on the wavelength scale in one, two or three dimensions. Due to interference effects, these structures can forbid light propagation over a large spectral range called "the photonic bandgap". Integrated optics is one of the potential applications of photonic crystals in which the information can be treated as a light signal that propagates through photonic crystal-based devices, such as filters, lasers, waveguides, bends and multiplexers. The photonic crystals structures studied for integrated optics applications consist of periodic arrays of holes etched through classical semiconducteur-based planar waveguides. For any practical application, the optical properties of the fabricated components have to be tuned by external means. In this thesis, we explore a possible approach to photonic crystal tuning : the infiltration of photoactive organic materials in the holes of these photonic structures. First we considered liquid crystals, which exhibit anisotropic optical properties like birefringence due to their long-range orientational order. This latter molecular organization can be modified by external factors, i.e. temperature and electric field. Nematic liquid crystals were infiltrated in the holes of the photonic crystals to tune their properties by changing the temperature and applying an electric field. Moreover, the organization of liquid crystals in the nanometric holes were studied by optical techniques. The demand for all-optical tunable devices in integrated optical circuits led us to consider the possibility of optically tuning the response of photonic crystal structures. For this purpose, we used photo-induced phase transitions of a liquid crystal blend doped with azobenzene photochromic molecules. Since the phase transitions in these mixtures depend on the concentration of the photochromic compound and on the temperature, we studied in detail the phase diagram of the blend. The last part of this thesis focused on one of the most innovative aspects in the domain of photonic crystal tuning : selective infiltration to locally modify the optical properties of the photonic structures. We used the photopolymerization of acrylate monomers by ultraviolet laser irradiation. Finally, with three clear examples, this thesis shows that organic photoactive materials are well-adapted to be combined with photonic crystals. We demonstrated that the obtained hybrid structures are very promising for integrated optics applications.

EPFL2009

Accordabilité de la réponse optique des cristaux photoniques par infiltration de matériaux organiques

Pascale El-Kallassi

EPFL2009