Focus: The interface between data collection and data processing in cryo-EM
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The high-pressure freezing (HPF) technique was applied to the cryo-immobilization of alginate gels and the quality of the freezing analyzed on a TEM by comparison of the segregation pattern of samples of decreasing thickness. Dynamic simulations of heat tr ...
Specimen charging under X-ray illumination is a well known phenomenon that can seriously obstruct the analysis of insulating samples. Synchrotron X-PEEM spectromicroscopy can reach a lateral resolution of 20 nm, 1-2 orders of magnitude larger than electron ...
TEM image simulations are used to couple the results from molecular dynamics (MD) simulations to experimental TEM images. In particular we apply this methodology to the study of defects produced during irradiation. MD simulations have shown that irradiatio ...
Structure and morphology of solid particles in precipitation from aqueous solutions correlate with formation (solution) conditions and thus reflect the growth mechanism. Calcium phosphate precipitation obtained from dilute aqueous solutions at room and bod ...
Crystal damage induced by irradiation is investigated using transmission electron microscopy (TEM) coupled to molecular dynamics (MD) calculations. The displacement cascades are simulated for energies ranging from 10 to 50 keV in Al, Ni and Cu and for time ...
Secretins, a superfamily of multimeric outer membrane proteins, mediate the transport of large macromolecules across the outer membrane of Gramnegative bacteria. Limited proteolysis of secretin PulD from the Klebsiella oxytoca pullulanase secretion pathway ...
The lens major intrinsic protein (MTP, AQP0) is known to function as a water and solute channel. However, MIP has also been reported to occur in close membrane contacts between lens fiber cells, indicating that it has adhesive properties in addition to its ...
In the absence of high-order aberrations, the lattice fringe technique should allow measurement of grain boundary rigid-body displacements to accuracies about an order of magnitude better than the point-to-point resolution of the transmission electron micr ...
A transmission electron microscopy of pyramidal inversion domains induced by Mg doping in MOVPE and bulk GaN is presented. Based on high resolution observations and EDX analysis, two atomic models are proposed for the Mg-rich (0001) inversion domain bounda ...
We explored degradation in electron-beam-pumped Zn1-xCdxSe/ZnSe laser structures by combining cathodoluminescence (CL) measurements in a scanning electron microscope with transmission electron microscopy. The rate of degradation, measured as the decrease o ...
