Publication
Structure of the Type VI Secretion System Contractile Sheath
Publications associées (38)
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Recently, single-particle cryo-electron microscopy emerged as a technique capable of determining protein structures at near-atomic resolution and resolving protein dynamics with a temporal resolution ranging from second to milliseconds. This thesis describ ...
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Duncan Thomas Lindsay Alexander, Chih-Ying Hsu, Bernat Mundet, Jean-Marc Triscone
Heterostructures consisting of SmNiO3 and NdNiO3 alternating layers with additional LaAlO3 spacer layers were grown and fully characterized by means of x-ray diffraction, atomic force microscopy, and scanning transmission electron microscopy. A change in t ...
Melville2024In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes
Observing the fast dynamics of nanoscale systems is crucial in order to understand and ultimately control their behavior. Characterizing these dynamic processes requires techniques with atomic spatial resolution and a temporal resolution that matches the t ...
EPFL2023Towards near-atomic resolution imaging of microsecond nanoscale dynamics
Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...
EPFL2023New Methods for the Integrative Dynamic Modeling of Biomolecular Structures
The true understanding of most cellular functions is only really achievable through the structural determination of their underlying macromolecular assemblies. However, their size, large number of individual components and metastable states make their eluc ...
EPFL2021High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica
Henning Paul-Julius Stahlberg, Jasenko Zivanov
Urease converts urea into ammonia and carbon dioxide and makes urea available as a nitrogen source for all forms of life except animals. In human bacterial pathogens, ureases also aid in the invasion of acidic environments such as the stomach by raising th ...
2020Will Cryo-Electron Microscopy Shift the Current Paradigm in Protein Structure Prediction?
Matteo Dal Peraro, Luciano Andres Abriata
Protein dynamics is undoubtedly a pervasive ingredient in all biological functions. However, structural biology has been strongly driven by a static-centered view of protein architecture. We argue that the recent advances of cryo-electron microscopy (EM) h ...
AMER CHEMICAL SOC2020Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells
Sergey Nazarov, Petr Leiman, Ricardo Cesar Guerrero Ferreira
Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteri ...
WILEY2019Cryo-EM structure of phosphodiesterase 6 reveals insights into the allosteric regulation of type I phosphodiesterases
Henning Paul-Julius Stahlberg, Andreas Engel
Cyclic nucleotide phosphodiesterases (PDEs) work in conjunction with adenylate/guanylate cyclases to regulate the key second messengers of G protein-coupled receptor signaling. Previous attempts to determine the full-length structure of PDE family members ...
American Association for the Advancement of Science (AAAS)2019Miniaturized Sample Preparation for Transmission Electron Microscopy
Henning Paul-Julius Stahlberg, Anastasia Syntychaki
Due to recent technological progress, cryo-electron microscopy (cryo-EM) is rapidly becoming a standard method for the structural analysis of protein complexes to atomic resolution. However, protein isolation techniques and sample preparation methods for E ...
MyJove Corporation2018