Interaction of complexes I, III, and IV within the bovine respirasome by single particle cryoelectron tomography
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Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exc ...
Hydrophobins self assemble into amphipathic films at hydrophobic-hydrophilic interfaces. These proteins are involved in a broad range of processes in fungal development. We have studied the conformational changes that accompany the self-assembly of the hyd ...
The authors report compelling evidence for the formation of a fourth type of helical secondary structure of a beta-peptide in MeOH, and this phenomenon occurs if each amino acid has a hydroxy group in the 2-position. 2D-NMR expts. of terminally-protected b ...
High signal-to-noise Fourier transform IR (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT3R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein ...
Carbamoyl phosphate (CP) has been implicated as an educt for the synthesis of the CO and CN ligands of the metal centre of [NiFe]-hydrogenases in Escherichia coli, since CP synthetase mutants (carAB) are unable to generate active hydrogenases due to a bloc ...
Rotationally resolved spectra in the 5 nu (1) region of (CH3OH)-C-13 reveal strong vibrational splittings, which by analogy to (CH3OH)-C-12, we assign to a resonance between 5 nu (1) and 4 nu (1)+nu (2). Accordingly, the vibrational dynamics on a subpicose ...
Cross-correlation between the fluctuations of 13Ca-Ha interactions affects the relaxation behavior of two-spin coherences (zero- and double-quantum coherences) involving the 13Ca nuclei of two successive amino acid residues. The cross-correlation rates are ...
A serotonin 5-HT3 receptor was functionally expressed to high levels and on a large scale in mammalian cells with the Semliki Forest virus system. Conditions were optimized to maximize detergent solubilization of the receptor, while preserving ligand bindi ...
To test the effect of NH-C(=S) groups on the stability of beta-peptide secondary structures, the authors have synthesized three beta-thiohexapeptide analogs I (X = S, Y = Z = O; X = Z = S, Y = O; X = Y = Z = S) of H-(beta-HVal-beta-HAla-beta-HLeu)2-OH I (X ...
The reversible, oriented immobilization of proteins on solid surfaces is a prerequisite for the investigation of mol. interactions and the controlled formation of supramol. assemblies. This paper describes a generally applicable method using a synthetic ch ...