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Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...
The conformations of melittin, porin, and lactose permease reconstituted in liposomal membranes were investigated by Raman spectroscopy, and the folding of membrane domains was predicted. These data resulted in consistent models for the insertion of the pr ...
A new method was presented to investigate the interactions between membrane receptors and their ligands in an artificial, reconstituted membrane system. Lipid bilayers were anchored via covalently attached thiolipids to TiO2/SiO2 waveguide surfaces to prod ...
Raman spectroscopy and x-ray diffraction are used to investigate the influence of surface charges on the structure of ionizable lipid membranes of dimyristoylmethylphosphatidic acid. The membrane surface charge d. is regulated by varying the pH of the aq. ...
Type III protein secretion (TTS) is catalyzed by translocases that span both membranes of Gram-negative bacteria. A hydrophilic TTS component homologous to F-1/V-1-ATPases is ubiquitous and essential for secretion. We show that hrcN encodes the putative TT ...
American Society for Biochemistry & Molecular Biology (ASBMB)2003
A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ by using integrated optics, thus avoiding the need for addnl. labels. Phospholipid bilayers were attached covalently to waveguid ...
How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological act ...
Aerolysin is one of a large group of bacterial proteins that can kill target cells by forming discrete channels in their plasma membranes. The toxin has many properties in common with the porins of the Gram-negative bacterial outer membrane, including an e ...
