Publication
Development of novel tools for imaging of metabolic fluxes in vivo
Publications associées (48)
Real-Time Bioluminescence Imaging of Glycans on Live Cells
Cell-surface glycans are attractive targets for molecule imaging due to their reflection of cellular processes associated with development and disease progression. In this paper, we describe the design, synthesis, and biological application of a new phosph ...
American Chemical Society2010Characterization of the erythrocyte response to glucose levels by impedance spectroscopy
Philippe Renaud, Thomas Michael Braschler, Guillaume Berlier
[...] For many years, researchers in the biosensors field have been trying to develop a device allowing the precise measurement of the glucose level through the intact skin. In this project, an efficient device was constructed for the in-vitro impedance me ...
2008Metabolic network analysis on Phaffia rhodozyma yeast using 13C-labeled glucose and gas chromatography-mass spectrometry
Urs von Stockar, Christopher Cannizzaro, Jens Nielsen
Carotenoid prodn. by microorganisms, as opposed to chem. synthesis, could fulfill an ever-increasing demand for 'all natural' products. The yeast Phaffia rhodozyma has received considerable attention because it produces the red pigment astaxanthin, commonl ...
2004Dynamic or inert metabolism? Turnover of N-acetyl aspartate and glutathione from D-[1-13C]glucose in the rat brain in vivo
The rate of (13)C-label incorporation into both aspartyl (NAA C3) and acetyl (NAA C6) groups of N-acetyl aspartate (NAA) was simultaneously measured in the rat brain in vivo for up to 19 h of [1-(13)C]glucose infusion (n = 8). Label incorporation was detec ...
Wiley-Blackwell2004Quantitative comparison of transient growth of Saccharomyces cerevisiae, Saccharomyces kluyveri, and Kluyveromyces lactis
Urs von Stockar, Christoph Herwig
A multitude of metabolic regulations occur in yeast, particularly under dynamic process conditions, such as under sudden glucose excess. However, quantification of regulations and classification of yeast strains under these conditions have yet to be elucid ...
2003Quantification and characterization of uncertainty in metabolic engineering
One of the central problems in metabolic engineering deals with the identification of the "rate limiting steps", i.e. enzymes whose activities one should manipulate towards the achievement of a desirable performance. However, uncertainty about the kinetic ...
2003The Effect of Insulin on in Vivo Cerebral Glucose Concentrations and Rates of Glucose Transport/Metabolism in Humans
The continuous delivery of glucose to the brain is critically important to the maintenance of normal metabolic function. However, elucidation of the hormonal regulation of in vivo cerebral glucose metabolism in humans has been limited by the lack of direct ...
2001In vivo measurements of brain glucose transport using the reversible michaelis-menten model and simultaneous measurements of cerebral blood flow changes during hypoglycemia
Glucose is the major substrate that sustains normal brain function. When the brain glucose concentration approaches zero, glucose transport across the blood-brain barrier becomes rate limiting for metabolism during, for example, increased metabolic activit ...
2001Cellular mechanisms of brain energy metabolism and their relevance to functional brain imaging
Pierre Magistretti, Luc Pellerin
Despite striking advances in functional brain imaging, the cellular and molecular mechanisms that underlie the signals detected by these techniques are still largely unknown. The basic physiological principle of functional imaging is represented by the tig ...
1999Metabolic coupling during activation: A cellular view
Pierre Magistretti, Luc Pellerin
A tight coupling exists between neuronal activity and energy metabolism. Over a century ago Roy and Sherrington postulated that “the brain possesses intrinsic mechanisms by which its vascular supply can be varied locally in correspondence with local variat ...
1997