Publication
Nanoliter plates—versatile tools for the screening of split-and-mix libraries on-bead and off-bead
Publications associées (31)
Integrated nanoreactor system
This thesis describes a method that allows the production in parallel of nano-reactor systems whose function is controlled by a remote stimulus. The reactors comprise a nested system of lipid vesicles part of which release their content during a thermotrop ...
EPFL2006Novel reactive perstraction system applied to the hydrolysis of penicillin G
Urs von Stockar, Ian William Marison
The activity of penicillin acylase has been studied in aq. and org. solvents, as free enzyme as well as immobilized within the membrane of liq.-core capsules. The activity of the enzyme is inhibited by the accumulation of the products of the hydrolysis rea ...
2005QM and QM/MM studies of the phosphoryl transfer reaction catalyzed by a cyclin-dependent kinase (CDKs)
The regulation of the eukaryotic cell cycle depends on the action of several related Ser/Thr protein kinases called cyclin-dependent kinases (CDKs), which are transiently activated at specific stages of the cycle, and which are also very promising targets ...
2004Substrate binding to mononuclear metallo-beta-lactamase from Bacillus cereus
Structure and dynamics of substrate binding (cefotaxime) to the catalytic pocket of the mononuclear zinc-beta-lactamase from Bacillus cereus are investigated by molecular dynamics simulations. The calculations, which are based on the hydrogen-bond pattern ...
2004Reaction Mechanism of HIV-1 Protease by Hybrid Car-Parrinello/Classical MD Simulations
Ursula Röthlisberger, Denis Bucher, Paolo Carloni
The authors present a QM/MM ab initio mol. dynamics study of the peptide hydrolysis reaction catalyzed by HIV-1 protease. The QM/MM calcns. are based on previous extensive classical MD simulations on the protein in complex with a model substrate. Gradient- ...
2004Protonation states of methionine aminopeptidase and their relevance for inhibitor binding and catalytic activity
The authors have performed a computational study of different protomeric states of the methionine aminopeptidase active site using a combined quantum-mech./mol. mech. simulation approach. The aim of this study was to clarify the native protonation state of ...
2003A short and flexible route to aza-beta-(1 -> 6)-C-disaccharides: Selective alpha-glycosidase inhibitors
The syntheses of azaMan-beta-(1-->6)-C-Glc (4), azaGlc-beta-(1-->6)-C-Glc (5), and azaGal-beta-(1-->6)-C-Glc (6) based upon double reductive amination of acetylenic carbohydrate-derived diketones is described. The required diketones are obtained by additio ...
1999Apparatus for enzymatic hydrolysis of proteins
A method and app. for enzymic hydrolysis of proteins comprises a 1st vessel in which the substrate and enzyme is mixed and the substrate undergoes a 1st hydrolysis. This hydrolyzate is subjected to a 2nd hydrolysis step in a tube or tubes contg. stationary ...
(Societe des Produits Nestle S. A., Switz.).1993Deactivation kinetics and temperature optimization of discontinuous enzymic processes
The influence of temp. on the catalytic activity and deactivation of sol. enzymes which are com. used in batch processes is discussed. Since high temp. not only accelerates the desired enzymic reaction but also the undesired deactivation of the enzyme, it ...
1985The influence of the ion content of whey on the pH-activity profile of the b-galactosidase from Aspergillus oryzae
The pH-dependence of the reaction kinetics of lactase (b-galactosidase) [9031-11-2] from A. oryzae in different reaction media is presented in terms of a 2-parameter model. The lactase from A. oryzae seems to have replaced the A. niger lactase on the marke ...
1985